Detailed information of TA system TA224622

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 74675..75349
• Replicon: plasmid P6
• Accession: NZ_CP086977
• Organism: Pseudosulfitobacter pseudonitzschiae strain SC1-114

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LOE10_RS23815
• Protein ID: WP_224557924.1
• Coordinates: 74927..75349 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LOE10_RS23810
• Protein ID: WP_229939240.1
• Coordinates: 74675..74923 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE10_RS23790 (LOE10_23775)	70588..71232	+	645	WP_224557929.1	type IV secretion system protein	-
LOE10_RS23795 (LOE10_23780)	71236..71967	+	732	WP_224557928.1	TrbG/VirB9 family P-type conjugative transfer protein	-
LOE10_RS23800 (LOE10_23785)	71970..73427	+	1458	WP_224557927.1	TrbI/VirB10 family protein	-
LOE10_RS23805 (LOE10_23790)	73538..74527	+	990	WP_224557926.1	P-type DNA transfer ATPase VirB11	-
LOE10_RS23810 (LOE10_23795)	74675..74923	+	249	WP_229939240.1	plasmid stabilization protein	Antitoxin
LOE10_RS23815 (LOE10_23800)	74927..75349	+	423	WP_224557924.1	type II toxin-antitoxin system VapC family toxin	Toxin
LOE10_RS23820 (LOE10_23805)	75346..78096	+	2751	WP_224557923.1	lactate dehydrogenase	-
LOE10_RS23825 (LOE10_23810)	78093..79151	+	1059	WP_224557922.1	HEPN domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..81284	81284

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15255.26 Da
• Isoelectric Point: 4.3767

>T224622 WP_224557924.1 NZ_CP086977:74927-75349 [Pseudosulfitobacter pseudonitzschiae]
MIILDTNVICELLRPTPEPRIEQWLSAQDGLNVYLTSISEAELRYGLANMGNGKRRAALIDAVDRILREDLAGRILPFDS
DAAQAYASIAATRRDAGRPIAQADCQIASIAHACGATVATRNTPDFEGCEIDLINPWTTE

Nucleotide

• Length: 423 bp

>T224622 NZ_CP120366:966300-966593 [Sinorhizobium kummerowiae]
ATGAGGCTCGTCTGGGCGCGATATGCGCTTGACGATCGCGACACCATCTTCAGTTACATCGAAAGGGAGAATCCGAGGGC
GGCGGTCCACGTCGACGAGGAGATCGTCAGCGCGGTACGTCGTCTGCTTGATTTTCCGGAAAGCGGCCGCCCTGGCCGGA
TTGCCGGAACTCGAGAGCTTGTTATCCCTCGGACCCCCTACATTGCAGCCTACATGGTAATGGAGGATAGAATTCGAATT
CTGCGTGTCCTGCACGGCGCCCAGAAGTGGCCCAGCGAGCTCGACGACGGGTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9206.46 Da
• Isoelectric Point: 6.7281

>AT224622 WP_229939240.1 NZ_CP086977:74675-74923 [Pseudosulfitobacter pseudonitzschiae]
MASITIRNLDDDVKRRLRIRAAEHGRSMEEEAREILRQVVGDAKPNHDLAAAIRARVGPLGGVELDLPQREAMHEPPTFD
RA

Nucleotide

• Length: 249 bp

>AT224622 NZ_CP120366:965995-966303 [Sinorhizobium kummerowiae]
ATGGCTTCAAATGCACTTGTTCAGACTCGAATCGATGCCGAGGTCAAAGAGCGCGCCACGGCGGTTTTAGAGAACATGGG
TCTGACGGTCTCGGACGCTGTTCGAATCCTGTTAACTCGGACGGCGAATGAGGGCGCCCTGCCGCTGGAACTCTTCAGCC
ACAGCGAGGCGCACGACGCTTGGTTCCGCGCCAAAGTGTTGCGGGCACTCGAGGATACCCGACCCGACGTCGACGATGCC
GACGCCGATGCGCACTTTCGAGAGCGCCGGGCGGCAGCTTTGCGCAAGGCTGCGGCGGGTGATCGATGA