Detailed information of TA system TA022462

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3370882..3371717
• Replicon: chromosome
• Accession: NC_009801
• Organism: Escherichia coli E24377A

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7W4PV54
• Locus tag: ECE24377A_RS18425
• Protein ID: WP_000854821.1
• Coordinates: 3371340..3371717 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A7ZRC5
• Locus tag: ECE24377A_RS18420
• Protein ID: WP_001285604.1
• Coordinates: 3370882..3371250 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECE24377A_RS18400	3368610..3369428	+	819	WP_001234386.1	DUF945 domain-containing protein	-
ECE24377A_RS18405	3369520..3370002	+	483	WP_000206657.1	antirestriction protein	-
ECE24377A_RS18410	3370018..3370494	+	477	WP_001384108.1	RadC family protein	-
ECE24377A_RS18415	3370581..3370802	+	222	WP_000692350.1	DUF987 domain-containing protein	-
ECE24377A_RS18420	3370882..3371250	+	369	WP_001285604.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
ECE24377A_RS18425	3371340..3371717	+	378	WP_000854821.1	type IV toxin-antitoxin system toxin CbtA	Toxin
ECE24377A_RS30150	3371714..3371911	+	198	Protein_3191	hypothetical protein	-
ECE24377A_RS30155	3371939..3372136	+	198	WP_085949090.1	DUF957 domain-containing protein	-
ECE24377A_RS18440	3372221..3373063	+	843	WP_001280493.1	DUF4942 domain-containing protein	-
ECE24377A_RS18445	3373401..3374459	-	1059	WP_000534925.1	site-specific integrase	-
ECE24377A_RS18450	3374501..3376156	-	1656	WP_000934715.1	TraI domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	gspM / gspL / gspK / gspJ / gspI / gspH / gspG / gspF / gspE / gspD / gspC / rfaE	3348871..3530406	181535

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13930.95 Da
• Isoelectric Point: 8.5163

>T22462 WP_000854821.1 NC_009801:3371340-3371717 [Escherichia coli O139:H28 str. E24377A]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T22462 NC_009801:3371340-3371717 [Escherichia coli O139:H28 str. E24377A]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13602.40 Da
• Isoelectric Point: 6.0618

>AT22462 WP_001285604.1 NC_009801:3370882-3371250 [Escherichia coli O139:H28 str. E24377A]
VSDTLPGTTLPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT22462 NC_009801:3370882-3371250 [Escherichia coli O139:H28 str. E24377A]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCCCCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7W4PV54

Antitoxin

• AlphaFold DB: A7ZRC5