Detailed information of TA system TA224616

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 545189..545654
• Replicon: chromosome
• Accession: NZ_CP086971
• Organism: Pseudosulfitobacter pseudonitzschiae strain SC1-114

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LOE10_RS02550
• Protein ID: WP_224557762.1
• Coordinates: 545391..545654 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LOE10_RS02545
• Protein ID: WP_224557764.1
• Coordinates: 545189..545404 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE10_RS02510 (LOE10_02510)	540297..540662	+	366	WP_028957811.1	cytochrome o ubiquinol oxidase subunit IV	-
LOE10_RS02515 (LOE10_02515)	540659..541357	+	699	WP_037931342.1	SURF1 family protein	-
LOE10_RS02525 (LOE10_02525)	541735..542919	+	1185	WP_224557768.1	site-specific integrase	-
LOE10_RS02530 (LOE10_02530)	542912..543883	+	972	WP_224557767.1	hypothetical protein	-
LOE10_RS02535 (LOE10_02535)	544064..544444	+	381	WP_224557766.1	helix-turn-helix domain-containing protein	-
LOE10_RS02540 (LOE10_02540)	544612..544836	+	225	WP_224557765.1	hypothetical protein	-
LOE10_RS02545 (LOE10_02545)	545189..545404	-	216	WP_224557764.1	CopG family antitoxin	Antitoxin
LOE10_RS02550 (LOE10_02550)	545391..545654	-	264	WP_224557762.1	BrnT family toxin	Toxin
LOE10_RS02555 (LOE10_02555)	545842..546285	+	444	WP_224557760.1	hypothetical protein	-
LOE10_RS02560 (LOE10_02560)	546378..547486	+	1109	Protein_501	IS110 family transposase	-
LOE10_RS02565 (LOE10_02565)	547712..548200	+	489	WP_229938596.1	DEAD/DEAH box helicase family protein	-
LOE10_RS02570 (LOE10_02570)	548124..548390	+	267	WP_229938971.1	type I restriction-modification enzyme R subunit C-terminal domain-containing protein	-
LOE10_RS02575 (LOE10_02575)	548387..549937	+	1551	WP_224557015.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-80)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10254.52 Da
• Isoelectric Point: 5.6550

>T224616 WP_224557762.1 NZ_CP086971:c545654-545391 [Pseudosulfitobacter pseudonitzschiae]
MKFEYDPDKSAGNKAKHGIDFDEAQALWEDPWLLEAPARTDDEPRFFAIGMIKERHWSAVCTYRGDIIRIISVRRARKQE
IEHYEST

Nucleotide

• Length: 264 bp

>T224616 NZ_CP120365:c3308276-3308025 [Sinorhizobium kummerowiae]
ATGAAGAAGGTCGTTTACAGCAAATCGTCGGTGAAGCTGCTCCGCCGCCTCGCGGCAAATGACGCGAAGCGCATTGTATC
GAAGGTCGAACAGTATGCGAGCGATCCAAAGTCTCTTGGGAACAACGTCAAGCCGCTGACCGGCTCGCCTTACATTCGCC
TCCGTGTTGGCGATTGGCGGGTTATCATGGACGATCAAGGTGTTGTCCTCGACATCATCAAGATCGGCTCTCGCGGCAGC
GTCTACGAATAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8265.40 Da
• Isoelectric Point: 10.6538

>AT224616 WP_224557764.1 NZ_CP086971:c545404-545189 [Pseudosulfitobacter pseudonitzschiae]
MKAREFDQKFDTGENVSDLVDWSKARRTNTQTKRVNVDFPSWVVSGLDQKAKQLGVTRQSLIKLWIAERLQ

Nucleotide

• Length: 216 bp

>AT224616 NZ_CP120365:c3308015-3307659 [Sinorhizobium kummerowiae]
ATGCAGACCATTACCACCCCGGGTGGCGAAACCCTGGTCGTCCTTCCCATTGCTGAATATGAAGATTTGCTCGATAGGGC
CGATATCGCGCAGGCCGACAAGGTGAAGGCCGATATCGCCGCCGGCCGCGACGAGCTCGTGCCGGCCGAGGTCGTCAACC
GTCTTCTGGCTGGCGAGAACGCGGTCAAGGTCTGGCGCTCCCGTCGCGGCATGGCCGCTCGAGAATTGGCGAAGAAGGCC
AGGATCAGCGCTCCCTATCTCTCCGAAATCGAGAGCGGGAAGAAGGACGGAAGTCTTTCAACCATGAAGAAGATTGCCGA
AGCTCTCTCCGTGGATCTCGACGATTTGGCGCAATAA