Detailed information of TA system TA224610

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 2246200..2246665
• Replicon: chromosome
• Accession: NZ_CP086963
• Organism: Pseudosulfitobacter pseudonitzschiae strain SC1-15

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LOE11_RS11065
• Protein ID: WP_224557762.1
• Coordinates: 2246402..2246665 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LOE11_RS11060
• Protein ID: WP_224557764.1
• Coordinates: 2246200..2246415 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE11_RS11025 (LOE11_11025)	2241308..2241673	+	366	WP_028957811.1	cytochrome o ubiquinol oxidase subunit IV	-
LOE11_RS11030 (LOE11_11030)	2241670..2242368	+	699	WP_037931342.1	SURF1 family protein	-
LOE11_RS11040 (LOE11_11040)	2242746..2243930	+	1185	WP_224557768.1	site-specific integrase	-
LOE11_RS11045 (LOE11_11045)	2243923..2244894	+	972	WP_224557767.1	hypothetical protein	-
LOE11_RS11050 (LOE11_11050)	2245075..2245455	+	381	WP_224557766.1	helix-turn-helix domain-containing protein	-
LOE11_RS11055 (LOE11_11055)	2245623..2245847	+	225	WP_224557765.1	hypothetical protein	-
LOE11_RS11060 (LOE11_11060)	2246200..2246415	-	216	WP_224557764.1	CopG family antitoxin	Antitoxin
LOE11_RS11065 (LOE11_11065)	2246402..2246665	-	264	WP_224557762.1	BrnT family toxin	Toxin
LOE11_RS11070 (LOE11_11070)	2246853..2247296	+	444	WP_224557760.1	hypothetical protein	-
LOE11_RS11075 (LOE11_11075)	2247389..2248498	+	1110	WP_224558030.1	IS110 family transposase	-
LOE11_RS11080 (LOE11_11080)	2248724..2249212	+	489	WP_229938596.1	DEAD/DEAH box helicase family protein	-
LOE11_RS11085 (LOE11_11085)	2249136..2249402	+	267	WP_229938971.1	type I restriction-modification enzyme R subunit C-terminal domain-containing protein	-
LOE11_RS11090 (LOE11_11090)	2249399..2250949	+	1551	WP_224557015.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2244100..2255259	11159

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-80)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10254.52 Da
• Isoelectric Point: 5.6550

>T224610 WP_224557762.1 NZ_CP086963:c2246665-2246402 [Pseudosulfitobacter pseudonitzschiae]
MKFEYDPDKSAGNKAKHGIDFDEAQALWEDPWLLEAPARTDDEPRFFAIGMIKERHWSAVCTYRGDIIRIISVRRARKQE
IEHYEST

Nucleotide

• Length: 264 bp

>T224610 NZ_CP120365:1370199-1370603 [Sinorhizobium kummerowiae]
TTGAACGGGTATCTTCTCGATACGAACATCATCAGCGACGTCATTCACAATCCGTTCGGACCGGCTGCGCAACGCATCGA
ACGGATCGGCCCGAAGGAGATTTACACCAGCATCGTCGTCGCGTCCGAGTTGCGGTATGGTTGCGCAAAAAAGGGATCGG
CGAAGTTGCTCGCCAAGGTCGAAAGCTTGCTGGAAATCGTCCCGGTGCTGCCATTGGATATTCCGGCCGACACCCGATAT
GGCAGCATTCGTGCGGAATTGGAGTCTCTGGGGCAGACCATCAGCTCCAACGATCTGTTGATTGCCGCGCATGCGTATGC
GCTGGACCTGACGCTGGTGACGGACAATATCCGTGAGTTCAGCCGCGTTCGTGGCCTTAGTCTGGAAAACTGGCTTGAGC
GATAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8265.40 Da
• Isoelectric Point: 10.6538

>AT224610 WP_224557764.1 NZ_CP086963:c2246415-2246200 [Pseudosulfitobacter pseudonitzschiae]
MKAREFDQKFDTGENVSDLVDWSKARRTNTQTKRVNVDFPSWVVSGLDQKAKQLGVTRQSLIKLWIAERLQ

Nucleotide

• Length: 216 bp

>AT224610 NZ_CP120365:1369930-1370202 [Sinorhizobium kummerowiae]
ATGCCCGTCCCGTTGCCGTCATCGCGACCTAAAGAAGTAAAGCTGTTCCGCAATAACCGCAGCCAGGCAGTCCGTATTCC
TGCGGAGTTCGAGTTGCCTGGAGACCGTGTGTTGATCCGTCGCGAAGGAACGAGACTCATTATCGAACCGATAGCCAGGC
CAGCCGATATCGTGGAGCTTCTCGCGGAGTGGAAGAAGGAAGCTCCCCTCGGACCTGAAGACCGGTTCCCGGATGTTGAG
GATATACCCGCCCGGCCGGAGAAAATCTTTTGA