Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3243877..3244531 Replicon chromosome
Accession NC_009801
Organism Escherichia coli E24377A

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag ECE24377A_RS17780 Protein ID WP_000244777.1
Coordinates 3243877..3244284 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag ECE24377A_RS17785 Protein ID WP_000354046.1
Coordinates 3244265..3244531 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECE24377A_RS17760 3239834..3241567 - 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -
ECE24377A_RS17765 3241573..3242283 - 711 WP_000715211.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
ECE24377A_RS17770 3242308..3243204 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
ECE24377A_RS17775 3243316..3243837 + 522 WP_001055874.1 flavodoxin FldB -
ECE24377A_RS17780 3243877..3244284 - 408 WP_000244777.1 toxin CptA Toxin
ECE24377A_RS17785 3244265..3244531 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
ECE24377A_RS17790 3244774..3245754 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
ECE24377A_RS17795 3245831..3246490 - 660 WP_000250274.1 hemolysin III family protein -
ECE24377A_RS17800 3246654..3246965 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
ECE24377A_RS17805 3247010..3248443 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
ECE24377A_RS17810 3248500..3249243 - 744 WP_000951948.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T22461 WP_000244777.1 NC_009801:c3244284-3243877 [Escherichia coli O139:H28 str. E24377A]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T22461 NC_009801:c3244284-3243877 [Escherichia coli O139:H28 str. E24377A]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT22461 WP_000354046.1 NC_009801:c3244531-3244265 [Escherichia coli O139:H28 str. E24377A]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT22461 NC_009801:c3244531-3244265 [Escherichia coli O139:H28 str. E24377A]
ATGGACATTAATAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References