Detailed information of TA system TA224608

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 14196..14870
• Replicon: plasmid P6
• Accession: NZ_CP086960
• Organism: Pseudosulfitobacter pseudonitzschiae strain SC1-27

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LOE13_RS22475
• Protein ID: WP_224557924.1
• Coordinates: 14448..14870 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LOE13_RS22470
• Protein ID: WP_229939240.1
• Coordinates: 14196..14444 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE13_RS22450 (LOE13_22435)	10109..10753	+	645	WP_224557929.1	type IV secretion system protein	-
LOE13_RS22455 (LOE13_22440)	10757..11488	+	732	WP_224557928.1	TrbG/VirB9 family P-type conjugative transfer protein	-
LOE13_RS22460 (LOE13_22445)	11491..12948	+	1458	WP_224557927.1	TrbI/VirB10 family protein	-
LOE13_RS22465 (LOE13_22450)	13059..14048	+	990	WP_224557926.1	P-type DNA transfer ATPase VirB11	-
LOE13_RS22470 (LOE13_22455)	14196..14444	+	249	WP_229939240.1	plasmid stabilization protein	Antitoxin
LOE13_RS22475 (LOE13_22460)	14448..14870	+	423	WP_224557924.1	type II toxin-antitoxin system VapC family toxin	Toxin
LOE13_RS22480 (LOE13_22465)	14867..17617	+	2751	WP_224557923.1	lactate dehydrogenase	-
LOE13_RS22485 (LOE13_22470)	17614..18672	+	1059	WP_224557922.1	HEPN domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..97185	97185

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15255.26 Da
• Isoelectric Point: 4.3767

>T224608 WP_224557924.1 NZ_CP086960:14448-14870 [Pseudosulfitobacter pseudonitzschiae]
MIILDTNVICELLRPTPEPRIEQWLSAQDGLNVYLTSISEAELRYGLANMGNGKRRAALIDAVDRILREDLAGRILPFDS
DAAQAYASIAATRRDAGRPIAQADCQIASIAHACGATVATRNTPDFEGCEIDLINPWTTE

Nucleotide

• Length: 423 bp

>T224608 NZ_CP120365:917283-917471 [Sinorhizobium kummerowiae]
GTGAAGAGCGGCGACATTATTGCAGCATTGCAGAAGGACGGATGGTACGAGGTTGCGACCAAGGGCAGCCACGTTCAATT
CAAACATCCGAAAAAGCACGGCCGGGTTACCGTTCCTCACCCGAAGAGGGATCTACCAATCGGTACCCTCAGGAGCATTG
AAAAGCAGTCCGGTTTGAAATTGAGGTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9206.46 Da
• Isoelectric Point: 6.7281

>AT224608 WP_229939240.1 NZ_CP086960:14196-14444 [Pseudosulfitobacter pseudonitzschiae]
MASITIRNLDDDVKRRLRIRAAEHGRSMEEEAREILRQVVGDAKPNHDLAAAIRARVGPLGGVELDLPQREAMHEPPTFD
RA

Nucleotide

• Length: 249 bp

>AT224608 NZ_CP120365:917476-917913 [Sinorhizobium kummerowiae]
ATGCGCAACTATATCGGATTGATCCACAAGGACGCCGAGAGCGATTACGGCGTTTCCTTTCCTGATTTTTCCGGCGTCGT
GACCGCCGGTGCCGACCTCGACGACGCGCGCGCTATGGCAGAGGAAGCCTTGGCCTTGCATATTGAAGGGCTGGTAGAGG
ACGGCGAGGCCATCCCGGAGCCCTCCTCCCTTGAAGTTGTCATGTCCGACGTCGAGAACAAGGATTGCGTCGCTATTCTC
GTCGCGGTGAAAACCGAGGCAAAAAGAGCCATTCGAGTGAACGTTACGCTTCCCGAAGGTGTCCTGAAGCAGATAGATGC
CTTCGCCGAAGCCCACGGCCTTACGCGGTCTGGATTTCTTGCTCGTGCTGCAACGCACGAGATCGAACGGGCAAATGACG
GCCACGACGCTTATGCGGAATCCCGCTTGTCGGCTTAG