Detailed information of TA system TA224606

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 22422..22983
• Replicon: plasmid P4
• Accession: NZ_CP086958
• Organism: Pseudosulfitobacter pseudonitzschiae strain SC1-27

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LOE13_RS21195
• Protein ID: WP_224557555.1
• Coordinates: 22422..22697 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LOE13_RS21200
• Protein ID: WP_224557554.1
• Coordinates: 22690..22983 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE13_RS21180 (LOE13_21170)	18211..19527	-	1317	WP_224557560.1	lactate racemase domain-containing protein	-
LOE13_RS21185 (LOE13_21175)	19544..21091	-	1548	WP_224557559.1	altronate dehydratase family protein	-
LOE13_RS21190 (LOE13_21180)	21216..21953	+	738	WP_229940771.1	GntR family transcriptional regulator	-
LOE13_RS21195 (LOE13_21185)	22422..22697	-	276	WP_224557555.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LOE13_RS21200 (LOE13_21190)	22690..22983	-	294	WP_224557554.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
LOE13_RS21205 (LOE13_21195)	23054..23959	-	906	WP_224557552.1	dihydrodipicolinate synthase family protein	-
LOE13_RS21210 (LOE13_21200)	23970..25709	-	1740	WP_224557550.1	L-arabinonate dehydratase	-
LOE13_RS21215 (LOE13_21205)	25971..27506	+	1536	WP_224557548.1	aldehyde dehydrogenase (NADP(+))	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..82230	82230

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-89)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-55)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10108.67 Da
• Isoelectric Point: 4.4912

>T224606 WP_224557555.1 NZ_CP086958:c22697-22422 [Pseudosulfitobacter pseudonitzschiae]
VPDLEWKALAVADLMAIVDYISDDNPDAAISLMDDIQDKVLGLLTHPKSGRPGRVNDTRELVIRPNYIVVYAENTAAVTI
LRVLHAVQMWP

Nucleotide

• Length: 276 bp

>T224606 NZ_CP120364:c1271841-1271398 [Sinorhizobium kummerowiae]
GTGACTTTCCTGCTCGACGTCAATGTGCTGATTGCCTTGATCGATCCGAGCCATATAGGCCATGATGATGCGCACGAATG
GTTCGCGTCGATTGGTCAGACCGCGTGGGCCACCTGCCCAATTACCGAAAATGGTGTCATCCGGATCGTCGGCAATCCCA
AATATCCCAATTCTCCCGGCTCACCGTTACCCGTGATGGAGATCGTCGCCAAATTGCGGTCGCTCCCCGGTCACGTTTTT
TGGCCGGACGATGTGAGCCTTGTCGGGTCAAGTGATATCATCCCATCGAAAATCCTGACTTCGGGGCAGGTGACTGACAC
CTATCTGCTTGCGCTCGCCAAGGTGCGTGGGGGCAAGCTGGCGACATTCGATCGCAAGCTGTCGGCAGCGGCGGTCACGA
AGGGCAATTCCGCCTTGCACCTGATCGCGACGAATAGGTCGTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10166.59 Da
• Isoelectric Point: 7.2024

>AT224606 WP_224557554.1 NZ_CP086958:c22983-22690 [Pseudosulfitobacter pseudonitzschiae]
MSAQTSMIHVRVDDQLKAKATDALSGVGLTLSDAVRILLTRVAAEGGLPAGLTANPDAYDAWFRAKVQDALADAAPAKPH
DQVMRDARALIDGKRGA

Nucleotide

• Length: 294 bp

>AT224606 NZ_CP120364:c1272065-1271838 [Sinorhizobium kummerowiae]
ATGAGAACGACCTTGGCTATTGACGACGATGTCCTGATCGCCGCAAAGGCAATGGCGACCCAACAGCAGCGGAGCGTCGG
CGAGGTAATTTCCGAACTGGCGAGGCGTTCTCTGCGCCGGCCGCGCAGCGGCGGCGAGCGTAATGGAATTCCTCTCCTGT
CGCCTCGTCCGGACGCACCGCCCGTGACGCTCGAGATCGTCAACGCGCTGCGTGACGAACTGGCGTGA