Detailed information of TA system TA224601

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 44387..44948
• Replicon: plasmid P5
• Accession: NZ_CP086952
• Organism: Pseudosulfitobacter pseudonitzschiae strain SC1-28

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LOE14_RS22550
• Protein ID: WP_224557555.1
• Coordinates: 44673..44948 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LOE14_RS22545
• Protein ID: WP_224557554.1
• Coordinates: 44387..44680 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE14_RS22530 (LOE14_22545)	39864..41399	-	1536	WP_224557548.1	aldehyde dehydrogenase (NADP(+))	-
LOE14_RS22535 (LOE14_22550)	41661..43400	+	1740	WP_224557550.1	L-arabinonate dehydratase	-
LOE14_RS22540 (LOE14_22555)	43411..44316	+	906	WP_224557552.1	dihydrodipicolinate synthase family protein	-
LOE14_RS22545 (LOE14_22560)	44387..44680	+	294	WP_224557554.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
LOE14_RS22550 (LOE14_22565)	44673..44948	+	276	WP_224557555.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LOE14_RS22555 (LOE14_22570)	45417..46187	-	771	WP_224557557.1	GntR family transcriptional regulator	-
LOE14_RS22560 (LOE14_22575)	46279..47826	+	1548	WP_224557559.1	altronate dehydratase family protein	-
LOE14_RS22565 (LOE14_22580)	47843..49159	+	1317	WP_224557560.1	lactate racemase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..82230	82230

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-89)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-55)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10108.67 Da
• Isoelectric Point: 4.4912

>T224601 WP_224557555.1 NZ_CP086952:44673-44948 [Pseudosulfitobacter pseudonitzschiae]
VPDLEWKALAVADLMAIVDYISDDNPDAAISLMDDIQDKVLGLLTHPKSGRPGRVNDTRELVIRPNYIVVYAENTAAVTI
LRVLHAVQMWP

Nucleotide

• Length: 276 bp

>T224601 NZ_CP120361:2150803-2151069 [Chelativorans sp. AA-79]
ATGGAGTTCGAATATGATCCGGAGAAGAGCGCTCGAAACCGCGCCAAACACGGCATTGATTTCGAGGAGGCACAGGCCCT
TTGGCTGGACGAGTGGCTGCTCGAAGTCTCGGCGCGAACGGTCGATGAGCCGCGGTATCTCGCGATCGGCACGTTGGGCG
GGAAGCATTGGACAGCGGTCTTCACGCGCAGAAACGGCCGGATAAGGCTTATGTCGGTTCGCTGCTCTCGGGAACAGGAA
GTCCGCCACTACGAGGACGTGATATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10166.59 Da
• Isoelectric Point: 7.2024

>AT224601 WP_224557554.1 NZ_CP086952:44387-44680 [Pseudosulfitobacter pseudonitzschiae]
MSAQTSMIHVRVDDQLKAKATDALSGVGLTLSDAVRILLTRVAAEGGLPAGLTANPDAYDAWFRAKVQDALADAAPAKPH
DQVMRDARALIDGKRGA

Nucleotide

• Length: 294 bp

>AT224601 NZ_CP120361:2151066-2151287 [Chelativorans sp. AA-79]
ATGAAAGTTAAAGCCGCAGAGTTCGATGAGGCCTTCGACGCAGGAGAGGATGTCTCCGATCTGGTTGATTGGGGCGCTTC
TCGACGGCCCAATCTGGAAACCAGAAGGGTGAACGTCGACTTTCCAGCCTGGGTCGTGGAAGGACTTGACCAGCAGGCGA
AGAAGCTGGGCGTCAGCCGCCAAGCTCTTATCAAATTATGGATTGCTGAGCGTCTGGGATAG