Detailed information of TA system TA224600

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 90587..91199
• Replicon: plasmid P4
• Accession: NZ_CP086951
• Organism: Pseudosulfitobacter pseudonitzschiae strain SC1-28

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LOE14_RS22250
• Protein ID: WP_028288627.1
• Coordinates: 90587..90982 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A921TG38
• Locus tag: LOE14_RS22255
• Protein ID: WP_009503866.1
• Coordinates: 90972..91199 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE14_RS22230 (LOE14_22245)	87106..87717	-	612	WP_197919471.1	SMC-Scp complex subunit ScpB	-
LOE14_RS22235 (LOE14_22250)	87765..88649	-	885	WP_037239259.1	DUF1403 family protein	-
LOE14_RS22240 (LOE14_22255)	88764..89915	+	1152	WP_224557873.1	tyrosine-type recombinase/integrase	-
LOE14_RS22245 (LOE14_22260)	90017..90508	+	492	Protein_92	recombinase family protein	-
LOE14_RS22250 (LOE14_22265)	90587..90982	-	396	WP_028288627.1	PIN domain-containing protein	Toxin
LOE14_RS22255 (LOE14_22270)	90972..91199	-	228	WP_009503866.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LOE14_RS22260 (LOE14_22275)	91408..92601	+	1194	WP_009503867.1	plasmid partitioning protein RepA	-
LOE14_RS22265 (LOE14_22280)	92585..93517	+	933	WP_028288626.1	plasmid partitioning protein RepB	-
LOE14_RS22270 (LOE14_22285)	93731..94942	+	1212	WP_050525470.1	plasmid replication protein RepC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..110570	110570

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-113)

Antitoxin

• Antitoxin PemI-like (2-47)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14212.23 Da
• Isoelectric Point: 4.1653

>T224600 WP_028288627.1 NZ_CP086951:c90982-90587 [Pseudosulfitobacter pseudonitzschiae]
MSVEFADTNVVLYLLDDGPKADRAEVILGQGPRISVQVLNESLVNCRRKAGLGWEEAAAFLEGVRALCPVEDLTVQTHDV
GRALAERYGFSIYDAMIVASALVAGCTTLWSEDMQDGLLVEGQLRIVNPFA

Nucleotide

• Length: 396 bp

>T224600 NZ_CP120361:c875789-875364 [Chelativorans sp. AA-79]
ATGCCCGATAGGCGGGGCGGCGCCCCTTCGAGCGCGTTCATCGATACGAATGTGATCCTCTATCTTGCCTCGGACGATCA
GCGGAAGGCTGAGCGGGCCGAAGGGATTGTAAGGCAGGGAGGCACCATAAGCGTCCAGGTGTTAAACGAGATCGCCAACG
CGGGGCGGCGCAAGATGCGCCTCGACTGGCAGCAGCTTCACGACTTCATAGATGCGATCCGCAGGCTTTTGAGGGTGGAG
AGCCTGACTGTCGATGTCCATGAAAGCGGAATGGCGATCGCTGAACGCTACGGAGTCTCCATCTATGACGGACTTATCGT
CGCTTCCGCCTTGCAGGCTGGCTGCACCATCCTTTGGTCGGAGGATATGCAAGACCGCCTGGCCATCGGGGACTTGCGGA
TCGCCAATCCATTCAGAACTTCATGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8535.79 Da
• Isoelectric Point: 11.0482

>AT224600 WP_009503866.1 NZ_CP086951:c91199-90972 [Pseudosulfitobacter pseudonitzschiae]
MQVAKWGNSLAVRLPAELVRELGLKEGDQIDLVKDDGRVRVRRLARADEVLTGLRRFRGKLSAAERLSRDDAHER

Nucleotide

• Length: 228 bp

>AT224600 NZ_CP120361:c876009-875779 [Chelativorans sp. AA-79]
ATGCGTGTGTCCAGGTGGGGCAACAGTCTTGCCATTCGCCTTCCCAGTTCGGTGGTCCAGCTTCTGAAGTTGGAGGAAGG
AGATGAAATTGAGTTGCACGCACAGGGAATGCGTTCGCTGGCGGTCGCGAAAAAACCTGAGCGCGAGGAGCTATTGACAC
GCCTGAGGCACTTCCGCGGCAAATTGCCTGCAGATTTCCGTTTCGACCGGGATGAGTTGAATGCCCGATAG