Detailed information of TA system TA224597

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 3787620..3788085
• Replicon: chromosome
• Accession: NZ_CP086947
• Organism: Pseudosulfitobacter pseudonitzschiae strain SC1-28

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LOE14_RS18875
• Protein ID: WP_224557762.1
• Coordinates: 3787822..3788085 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LOE14_RS18870
• Protein ID: WP_224557764.1
• Coordinates: 3787620..3787835 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE14_RS18835 (LOE14_18840)	3782728..3783093	+	366	WP_028957811.1	cytochrome o ubiquinol oxidase subunit IV	-
LOE14_RS18840 (LOE14_18845)	3783090..3783788	+	699	WP_037931342.1	SURF1 family protein	-
LOE14_RS18850 (LOE14_18855)	3784166..3785350	+	1185	WP_224557768.1	site-specific integrase	-
LOE14_RS18855 (LOE14_18860)	3785343..3786314	+	972	WP_224557767.1	hypothetical protein	-
LOE14_RS18860 (LOE14_18865)	3786495..3786875	+	381	WP_224557766.1	helix-turn-helix domain-containing protein	-
LOE14_RS18865 (LOE14_18870)	3787043..3787267	+	225	WP_224557765.1	hypothetical protein	-
LOE14_RS18870 (LOE14_18875)	3787620..3787835	-	216	WP_224557764.1	CopG family antitoxin	Antitoxin
LOE14_RS18875 (LOE14_18880)	3787822..3788085	-	264	WP_224557762.1	BrnT family toxin	Toxin
LOE14_RS18880 (LOE14_18885)	3788273..3788716	+	444	WP_224557760.1	hypothetical protein	-
LOE14_RS18885 (LOE14_18890)	3788809..3789918	+	1110	WP_224558030.1	IS110 family transposase	-
LOE14_RS18890 (LOE14_18895)	3790144..3790632	+	489	WP_229938596.1	DEAD/DEAH box helicase family protein	-
LOE14_RS18895 (LOE14_18900)	3790556..3790822	+	267	WP_229938971.1	type I restriction-modification enzyme R subunit C-terminal domain-containing protein	-
LOE14_RS18900 (LOE14_18905)	3790819..3792369	+	1551	WP_224557015.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3785520..3796679	11159

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-80)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10254.52 Da
• Isoelectric Point: 5.6550

>T224597 WP_224557762.1 NZ_CP086947:c3788085-3787822 [Pseudosulfitobacter pseudonitzschiae]
MKFEYDPDKSAGNKAKHGIDFDEAQALWEDPWLLEAPARTDDEPRFFAIGMIKERHWSAVCTYRGDIIRIISVRRARKQE
IEHYEST

Nucleotide

• Length: 264 bp

>T224597 NZ_CP120359:4565517-4565813 [Pseudomonas sp. AA-38]
ATGGCTGAAATAGTCTGGACGAATCCCGCCCTCGAACAACTCGACGAGCTGGCGCACTACATCGCTCTCGACAAACCCGA
CGCCGCTCGCGCGTTGGTCAGGAAGGTCGTCGAAGCCGTCGCGCGCCTGGCGGAGTTCCCCTTGTCAGGCCGGGTTCCTG
GCGAGCTACCGGACTCCGTGTATCGCGAAATCGTCGTCCCGCCCTGTCGGGTCTTCTACCGCCCGGCAGAGGGCAAGGTG
TTCATCATCCACATCATGCGAGAAGAGCGCCTGCTGCGCGCTCATCTGCTCGAGTGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8265.40 Da
• Isoelectric Point: 10.6538

>AT224597 WP_224557764.1 NZ_CP086947:c3787835-3787620 [Pseudosulfitobacter pseudonitzschiae]
MKAREFDQKFDTGENVSDLVDWSKARRTNTQTKRVNVDFPSWVVSGLDQKAKQLGVTRQSLIKLWIAERLQ

Nucleotide

• Length: 216 bp

>AT224597 NZ_CP120359:4565272-4565529 [Pseudomonas sp. AA-38]
ATGAAAGTCGAACTGGTCACCAACCTGAAGCGACAAGCCACCAAGATTCTCGCCGACCTTCACGAGACCAAAGAGCCCGT
GCTGATCACCGAGCATGGAGTGCCCTCGGCCTATCTGGTGGATGTCGATGATTACCAGTTCATGCAGAGGCGCCTCGCCC
TTCTGGATGCCCTGGCCTGCGGTGAACGGGCCGTACTGGAGGGCAATACCCTCAGCCATGAACAGGCCAGGGAACGGCTG
GGCAAATGGCTGAAATAG