Detailed information of TA system TA224595

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 76812..77486
• Replicon: plasmid P7
• Accession: NZ_CP086945
• Organism: Pseudosulfitobacter pseudonitzschiae strain SC1-29

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LOE15_RS22700
• Protein ID: WP_224557924.1
• Coordinates: 77064..77486 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LOE15_RS22695
• Protein ID: WP_229939240.1
• Coordinates: 76812..77060 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE15_RS22675 (LOE15_22635)	72725..73369	+	645	WP_224557929.1	type IV secretion system protein	-
LOE15_RS22680 (LOE15_22640)	73373..74104	+	732	WP_224557928.1	TrbG/VirB9 family P-type conjugative transfer protein	-
LOE15_RS22685 (LOE15_22645)	74107..75564	+	1458	WP_224557927.1	TrbI/VirB10 family protein	-
LOE15_RS22690 (LOE15_22650)	75675..76664	+	990	WP_224557926.1	P-type DNA transfer ATPase VirB11	-
LOE15_RS22695 (LOE15_22655)	76812..77060	+	249	WP_229939240.1	plasmid stabilization protein	Antitoxin
LOE15_RS22700 (LOE15_22660)	77064..77486	+	423	WP_224557924.1	type II toxin-antitoxin system VapC family toxin	Toxin
LOE15_RS22705 (LOE15_22665)	77483..80233	+	2751	WP_224557923.1	lactate dehydrogenase	-
LOE15_RS22710 (LOE15_22670)	80230..81288	+	1059	WP_224557922.1	HEPN domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..157664	157664

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15255.26 Da
• Isoelectric Point: 4.3767

>T224595 WP_224557924.1 NZ_CP086945:77064-77486 [Pseudosulfitobacter pseudonitzschiae]
MIILDTNVICELLRPTPEPRIEQWLSAQDGLNVYLTSISEAELRYGLANMGNGKRRAALIDAVDRILREDLAGRILPFDS
DAAQAYASIAATRRDAGRPIAQADCQIASIAHACGATVATRNTPDFEGCEIDLINPWTTE

Nucleotide

• Length: 423 bp

>T224595 NZ_CP120359:3256514-3256792 [Pseudomonas sp. AA-38]
ATGATCAAATCTTTCCAGCACAAAGGGCTGCGCAAGCTATTTGAAACGGGCAGCACAGCAGGCGTACAACCCGCCCATGC
CAAGCGGCTACGAATGCAGCTAGCAGCCCTGGATACAGCCATGACGGTAGACGACATGGACATCCCTGGCTTCCGCCTGC
ACCCGCTCAAGGGAGAAATGCGTGGGCGCTGGTCGATCATGGTGAACGGCAACTGGCGGCTGACGTTCGAGTTTCGCGAC
GGAAACGCTTATGTCCTGGACTATGAGGATTACCACTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9206.46 Da
• Isoelectric Point: 6.7281

>AT224595 WP_229939240.1 NZ_CP086945:76812-77060 [Pseudosulfitobacter pseudonitzschiae]
MASITIRNLDDDVKRRLRIRAAEHGRSMEEEAREILRQVVGDAKPNHDLAAAIRARVGPLGGVELDLPQREAMHEPPTFD
RA

Nucleotide

• Length: 249 bp

>AT224595 NZ_CP120359:3256792-3257079 [Pseudomonas sp. AA-38]
ATGAACATGCACAACCCGCCCCATCCTGGTGAATTCATCACCGATATCTACCTGGAGCCGAACGGCATCAGCGGTCGTGA
GCTGGCCGAAAAGCTGCGCGTAGCACCCTCGACCCTGAGCCGTGTGCTCAAGGGCACCAGCCGCGTAACGCCGGAAATGG
CTCTGCGCCTCTCGGTAGCACTTGGTCGCTCGCCTGAGAGCTGGCTAGCTATGCAAGATGCTTACGACTTATGGAATGCC
CGTCAGCATGTAGATCTGCAGCAGGTTGGAAAGCTGGCGTTTGCATGA