22459

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 2746685..2747310 Replicon chromosome
Accession NC_009801
Organism Escherichia coli E24377A

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag ECE24377A_RS15360 Protein ID WP_000911329.1
Coordinates 2746685..2747083 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag ECE24377A_RS15365 Protein ID WP_000450524.1
Coordinates 2747083..2747310 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECE24377A_RS15340 2742592..2742792 + 201 WP_000383836.1 YpfN family protein -
ECE24377A_RS15345 2742873..2743571 - 699 WP_000679795.1 esterase -
ECE24377A_RS15350 2743645..2745660 - 2016 WP_000829344.1 tRNA cytosine(34) acetyltransferase TmcA -
ECE24377A_RS15355 2745675..2746538 - 864 WP_001267507.1 neutral zinc metallopeptidase -
ECE24377A_RS15360 2746685..2747083 - 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
ECE24377A_RS15365 2747083..2747310 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
ECE24377A_RS15370 2747464..2748177 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
ECE24377A_RS15380 2748390..2749424 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
ECE24377A_RS15385 2749441..2750319 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
ECE24377A_RS15390 2750465..2751037 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
ECE24377A_RS15395 2751037..2751507 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T22459 WP_000911329.1 NC_009801:c2747083-2746685 [Escherichia coli O139:H28 str. E24377A]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T22459 NC_009801:c2747083-2746685 [Escherichia coli O139:H28 str. E24377A]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTAACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT22459 WP_000450524.1 NC_009801:c2747310-2747083 [Escherichia coli O139:H28 str. E24377A]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT22459 NC_009801:c2747310-2747083 [Escherichia coli O139:H28 str. E24377A]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References