Detailed information of TA system TA224582

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 170739..171280
• Replicon: plasmid P9
• Accession: NZ_CP086928
• Organism: Pseudosulfitobacter pseudonitzschiae strain SC1-32

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A073IW87
• Locus tag: LOE17_RS24715
• Protein ID: WP_037931244.1
• Coordinates: 170954..171280 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A073IWP4
• Locus tag: LOE17_RS24710
• Protein ID: WP_037931247.1
• Coordinates: 170739..170957 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE17_RS24690 (LOE17_24665)	165996..167003	+	1008	WP_081855484.1	PaaX family transcriptional regulator C-terminal domain-containing protein	-
LOE17_RS24695 (LOE17_24670)	167238..168785	+	1548	WP_073194592.1	AMP-binding protein	-
LOE17_RS24700 (LOE17_24675)	168840..169304	+	465	WP_051694690.1	MaoC/PaaZ C-terminal domain-containing protein	-
LOE17_RS24705 (LOE17_24680)	169308..170441	+	1134	WP_037931249.1	alpha/beta fold hydrolase	-
LOE17_RS24710 (LOE17_24685)	170739..170957	+	219	WP_037931247.1	antitoxin MazE family protein	Antitoxin
LOE17_RS24715 (LOE17_24690)	170954..171280	+	327	WP_037931244.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LOE17_RS24720 (LOE17_24695)	171454..171810	-	357	Protein_162	ATP-binding protein	-
LOE17_RS24725 (LOE17_24700)	171800..173372	-	1573	Protein_163	IS21 family transposase	-
LOE17_RS24730 (LOE17_24705)	173708..174031	-	324	WP_027238707.1	helix-turn-helix domain-containing protein	-
LOE17_RS24735 (LOE17_24710)	174194..174601	+	408	WP_157802081.1	DoxX family protein	-
LOE17_RS24740 (LOE17_24715)	174665..175480	+	816	WP_224556226.1	class III extradiol ring-cleavage dioxygenase	-
LOE17_RS24745 (LOE17_24720)	175489..175986	+	498	WP_037931962.1	DUF5519 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..193372	193372
	-	flank	IS/Tn	-	-	171800..173086	1286

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-105)

Antitoxin

• Antitoxin MazE-like (7-71)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11610.50 Da
• Isoelectric Point: 8.8545

>T224582 WP_037931244.1 NZ_CP086928:170954-171280 [Pseudosulfitobacter pseudonitzschiae]
VRRGELVTVALQGDHGKPRPALVIQSDRFPDTATTTVLLVTSTLVDAPLIRLTVEPSAENGLRMRSQIMIDKAVTVRTDK
LGKAFGQLDDTAMIEVNRALALFLGVAG

Nucleotide

• Length: 327 bp

>T224582 NZ_CP120349:c4216824-4216540 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTCGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8175.17 Da
• Isoelectric Point: 5.8017

>AT224582 WP_037931247.1 NZ_CP086928:170739-170957 [Pseudosulfitobacter pseudonitzschiae]
MPTSVSHRVQKRRDALRAAGLRPVQIWVPDTRRPGFEAECRRQAAIVAKADRADDTLNDFLDASLVDVDDWE

Nucleotide

• Length: 219 bp

>AT224582 NZ_CP120349:c4217056-4216814 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Toxin

• AlphaFold DB: A0A073IW87

Antitoxin

• AlphaFold DB: A0A073IWP4