Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/PemK-MazE |
| Location | 11527..12068 | Replicon | plasmid P2 |
| Accession | NZ_CP086911 | ||
| Organism | Pseudosulfitobacter pseudonitzschiae strain SC1-34 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | A0A073IW87 |
| Locus tag | LOE18_RS19655 | Protein ID | WP_037931244.1 |
| Coordinates | 11527..11853 (-) | Length | 109 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | A0A073IWP4 |
| Locus tag | LOE18_RS19660 | Protein ID | WP_037931247.1 |
| Coordinates | 11850..12068 (-) | Length | 73 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LOE18_RS19625 (LOE18_19590) | 6821..7318 | - | 498 | WP_037931962.1 | DUF5519 family protein | - |
| LOE18_RS19630 (LOE18_19595) | 7327..8142 | - | 816 | WP_224556226.1 | class III extradiol ring-cleavage dioxygenase | - |
| LOE18_RS19635 (LOE18_19600) | 8206..8613 | - | 408 | WP_157802081.1 | DoxX family protein | - |
| LOE18_RS19640 (LOE18_19605) | 8776..9099 | + | 324 | WP_027238707.1 | helix-turn-helix domain-containing protein | - |
| LOE18_RS19645 (LOE18_19610) | 9435..11007 | + | 1573 | Protein_13 | IS21 family transposase | - |
| LOE18_RS19650 (LOE18_19615) | 10997..11353 | + | 357 | Protein_14 | ATP-binding protein | - |
| LOE18_RS19655 (LOE18_19620) | 11527..11853 | - | 327 | WP_037931244.1 | type II toxin-antitoxin system PemK/MazF family toxin | Toxin |
| LOE18_RS19660 (LOE18_19625) | 11850..12068 | - | 219 | WP_037931247.1 | antitoxin MazE family protein | Antitoxin |
| LOE18_RS19665 (LOE18_19630) | 12366..13499 | - | 1134 | WP_037931249.1 | alpha/beta fold hydrolase | - |
| LOE18_RS19670 (LOE18_19635) | 13503..13967 | - | 465 | WP_051694690.1 | MaoC/PaaZ C-terminal domain-containing protein | - |
| LOE18_RS19675 (LOE18_19640) | 14022..15569 | - | 1548 | WP_073194592.1 | AMP-binding protein | - |
| LOE18_RS19680 (LOE18_19645) | 15804..16811 | - | 1008 | WP_081855484.1 | PaaX family transcriptional regulator C-terminal domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..182821 | 182821 | |
| - | flank | IS/Tn | - | - | 9721..11007 | 1286 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 109 a.a. Molecular weight: 11610.50 Da Isoelectric Point: 8.8545
>T224570 WP_037931244.1 NZ_CP086911:c11853-11527 [Pseudosulfitobacter pseudonitzschiae]
VRRGELVTVALQGDHGKPRPALVIQSDRFPDTATTTVLLVTSTLVDAPLIRLTVEPSAENGLRMRSQIMIDKAVTVRTDK
LGKAFGQLDDTAMIEVNRALALFLGVAG
VRRGELVTVALQGDHGKPRPALVIQSDRFPDTATTTVLLVTSTLVDAPLIRLTVEPSAENGLRMRSQIMIDKAVTVRTDK
LGKAFGQLDDTAMIEVNRALALFLGVAG
Download Length: 327 bp
>T224570 NZ_CP120347:4667743-4668054 [Salmonella enterica subsp. enterica serovar Muenchen]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATACTATCGGGGTAATAAAGAATGA
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATACTATCGGGGTAATAAAGAATGA
Antitoxin
Download Length: 73 a.a. Molecular weight: 8175.17 Da Isoelectric Point: 5.8017
>AT224570 WP_037931247.1 NZ_CP086911:c12068-11850 [Pseudosulfitobacter pseudonitzschiae]
MPTSVSHRVQKRRDALRAAGLRPVQIWVPDTRRPGFEAECRRQAAIVAKADRADDTLNDFLDASLVDVDDWE
MPTSVSHRVQKRRDALRAAGLRPVQIWVPDTRRPGFEAECRRQAAIVAKADRADDTLNDFLDASLVDVDDWE
Download Length: 219 bp
>AT224570 NZ_CP120347:4668051-4668497 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAACCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAATCACTCAAC
CAACACCTGGAGAAAACACCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTTAAACTACCAGCGGATGCCTTCATCGAGTAG
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAACCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAATCACTCAAC
CAACACCTGGAGAAAACACCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTTAAACTACCAGCGGATGCCTTCATCGAGTAG
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A073IW87 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A073IWP4 |