Detailed information of TA system TA224568

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 986053..986518
• Replicon: chromosome
• Accession: NZ_CP086909
• Organism: Pseudosulfitobacter pseudonitzschiae strain SC1-34

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LOE18_RS04980
• Protein ID: WP_224557762.1
• Coordinates: 986053..986316 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LOE18_RS04985
• Protein ID: WP_224557764.1
• Coordinates: 986303..986518 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE18_RS04955 (LOE18_04955)	981769..983319	-	1551	WP_224557015.1	restriction endonuclease subunit S	-
LOE18_RS04960 (LOE18_04960)	983316..983612	-	297	WP_224557153.1	type I restriction-modification enzyme R subunit C-terminal domain-containing protein	-
LOE18_RS04965 (LOE18_04965)	983506..983994	-	489	WP_229938596.1	DEAD/DEAH box helicase family protein	-
LOE18_RS04970 (LOE18_04970)	984220..985329	-	1110	WP_224558030.1	IS110 family transposase	-
LOE18_RS04975 (LOE18_04975)	985422..985865	-	444	WP_224557760.1	hypothetical protein	-
LOE18_RS04980 (LOE18_04980)	986053..986316	+	264	WP_224557762.1	BrnT family toxin	Toxin
LOE18_RS04985 (LOE18_04985)	986303..986518	+	216	WP_224557764.1	CopG family antitoxin	Antitoxin
LOE18_RS04990 (LOE18_04990)	986871..987095	-	225	WP_224557765.1	hypothetical protein	-
LOE18_RS04995 (LOE18_04995)	987263..987643	-	381	WP_224557766.1	helix-turn-helix domain-containing protein	-
LOE18_RS05000 (LOE18_05000)	987824..988795	-	972	WP_224557767.1	hypothetical protein	-
LOE18_RS05005 (LOE18_05005)	988788..989972	-	1185	WP_224557768.1	site-specific integrase	-
LOE18_RS05015 (LOE18_05015)	990350..991048	-	699	WP_037931342.1	SURF1 family protein	-
LOE18_RS05020 (LOE18_05020)	991045..991410	-	366	WP_028957811.1	cytochrome o ubiquinol oxidase subunit IV	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	977672..989972	12300

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-80)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10254.52 Da
• Isoelectric Point: 5.6550

>T224568 WP_224557762.1 NZ_CP086909:986053-986316 [Pseudosulfitobacter pseudonitzschiae]
MKFEYDPDKSAGNKAKHGIDFDEAQALWEDPWLLEAPARTDDEPRFFAIGMIKERHWSAVCTYRGDIIRIISVRRARKQE
IEHYEST

Nucleotide

• Length: 264 bp

>T224568 NZ_CP120347:c4216824-4216540 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTCGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8265.40 Da
• Isoelectric Point: 10.6538

>AT224568 WP_224557764.1 NZ_CP086909:986303-986518 [Pseudosulfitobacter pseudonitzschiae]
MKAREFDQKFDTGENVSDLVDWSKARRTNTQTKRVNVDFPSWVVSGLDQKAKQLGVTRQSLIKLWIAERLQ

Nucleotide

• Length: 216 bp

>AT224568 NZ_CP120347:c4217056-4216814 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA