Detailed information of TA system TA224562

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 2250670..2251135
• Replicon: chromosome
• Accession: NZ_CP086901
• Organism: Pseudosulfitobacter pseudonitzschiae strain SC1-35

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LOE19_RS11090
• Protein ID: WP_224557762.1
• Coordinates: 2250872..2251135 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LOE19_RS11085
• Protein ID: WP_224557764.1
• Coordinates: 2250670..2250885 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE19_RS11050 (LOE19_11060)	2245778..2246143	+	366	WP_028957811.1	cytochrome o ubiquinol oxidase subunit IV	-
LOE19_RS11055 (LOE19_11065)	2246140..2246838	+	699	WP_037931342.1	SURF1 family protein	-
LOE19_RS11065 (LOE19_11075)	2247216..2248400	+	1185	WP_224557768.1	site-specific integrase	-
LOE19_RS11070 (LOE19_11080)	2248393..2249364	+	972	WP_224557767.1	hypothetical protein	-
LOE19_RS11075 (LOE19_11085)	2249545..2249925	+	381	WP_224557766.1	helix-turn-helix domain-containing protein	-
LOE19_RS11080 (LOE19_11090)	2250093..2250317	+	225	WP_224557765.1	hypothetical protein	-
LOE19_RS11085 (LOE19_11095)	2250670..2250885	-	216	WP_224557764.1	CopG family antitoxin	Antitoxin
LOE19_RS11090 (LOE19_11100)	2250872..2251135	-	264	WP_224557762.1	BrnT family toxin	Toxin
LOE19_RS11095 (LOE19_11105)	2251323..2251766	+	444	WP_224557760.1	hypothetical protein	-
LOE19_RS11100 (LOE19_11110)	2251859..2252968	+	1110	WP_224558030.1	IS110 family transposase	-
LOE19_RS11105 (LOE19_11115)	2253194..2253682	+	489	WP_229938596.1	DEAD/DEAH box helicase family protein	-
LOE19_RS11110 (LOE19_11120)	2253606..2253872	+	267	WP_229938971.1	type I restriction-modification enzyme R subunit C-terminal domain-containing protein	-
LOE19_RS11115 (LOE19_11125)	2253869..2255419	+	1551	WP_224557015.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2248570..2259729	11159

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-80)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10254.52 Da
• Isoelectric Point: 5.6550

>T224562 WP_224557762.1 NZ_CP086901:c2251135-2250872 [Pseudosulfitobacter pseudonitzschiae]
MKFEYDPDKSAGNKAKHGIDFDEAQALWEDPWLLEAPARTDDEPRFFAIGMIKERHWSAVCTYRGDIIRIISVRRARKQE
IEHYEST

Nucleotide

• Length: 264 bp

>T224562 NZ_CP120347:854961-855374 [Salmonella enterica subsp. enterica serovar Muenchen]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTAATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGTTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8265.40 Da
• Isoelectric Point: 10.6538

>AT224562 WP_224557764.1 NZ_CP086901:c2250885-2250670 [Pseudosulfitobacter pseudonitzschiae]
MKAREFDQKFDTGENVSDLVDWSKARRTNTQTKRVNVDFPSWVVSGLDQKAKQLGVTRQSLIKLWIAERLQ

Nucleotide

• Length: 216 bp

>AT224562 NZ_CP120347:854714-854980 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA