Detailed information of TA system TA224560

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 225439..225980
• Replicon: plasmid P6
• Accession: NZ_CP086900
• Organism: Pseudosulfitobacter pseudonitzschiae strain SC1-37

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A073IW87
• Locus tag: LOE20_RS23980
• Protein ID: WP_037931244.1
• Coordinates: 225439..225765 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A073IWP4
• Locus tag: LOE20_RS23985
• Protein ID: WP_037931247.1
• Coordinates: 225762..225980 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE20_RS23950 (LOE20_23950)	220733..221230	-	498	WP_037931962.1	DUF5519 family protein	-
LOE20_RS23955 (LOE20_23955)	221239..222054	-	816	WP_224556226.1	class III extradiol ring-cleavage dioxygenase	-
LOE20_RS23960 (LOE20_23960)	222118..222525	-	408	WP_157802081.1	DoxX family protein	-
LOE20_RS23965 (LOE20_23965)	222688..223011	+	324	WP_027238707.1	helix-turn-helix domain-containing protein	-
LOE20_RS23970 (LOE20_23970)	223347..224919	+	1573	Protein_218	IS21 family transposase	-
LOE20_RS23975 (LOE20_23975)	224909..225265	+	357	Protein_219	ATP-binding protein	-
LOE20_RS23980 (LOE20_23980)	225439..225765	-	327	WP_037931244.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LOE20_RS23985 (LOE20_23985)	225762..225980	-	219	WP_037931247.1	antitoxin MazE family protein	Antitoxin
LOE20_RS23990 (LOE20_23990)	226278..227411	-	1134	WP_037931249.1	alpha/beta fold hydrolase	-
LOE20_RS23995 (LOE20_23995)	227415..227879	-	465	WP_051694690.1	MaoC/PaaZ C-terminal domain-containing protein	-
LOE20_RS24000 (LOE20_24000)	227934..229481	-	1548	WP_073194592.1	AMP-binding protein	-
LOE20_RS24005 (LOE20_24005)	229716..230723	-	1008	WP_081855484.1	PaaX family transcriptional regulator C-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..290597	290597
	-	flank	IS/Tn	-	-	223633..224919	1286

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-105)

Antitoxin

• Antitoxin MazE-like (7-71)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11610.50 Da
• Isoelectric Point: 8.8545

>T224560 WP_037931244.1 NZ_CP086900:c225765-225439 [Pseudosulfitobacter pseudonitzschiae]
VRRGELVTVALQGDHGKPRPALVIQSDRFPDTATTTVLLVTSTLVDAPLIRLTVEPSAENGLRMRSQIMIDKAVTVRTDK
LGKAFGQLDDTAMIEVNRALALFLGVAG

Nucleotide

• Length: 327 bp

>T224560 NZ_CP120346:50118-50423 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTACCGCCTGTTTGTGGATGTGCAGAGCGACATCGTCGATAC
CCCTGGCCGGCGAATGGTGATCCCGCTGGCCAGTGCCCGTCTGCTGTCGGATAAGGTCTCCCGCGAACTTTATCCGGTGG
TGCATATCGGGGATGAGAGCTGGCGTCTGATGACCACGGATATGGCCAGTGTCCCGGCCTCATTCATCGGGGAAGAGGTG
GCCGAGCTCAGCCACAGGGAGAACGACATCAAAAACGCCATTAACCTGATGTTCTGGGGGATCTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8175.17 Da
• Isoelectric Point: 5.8017

>AT224560 WP_037931247.1 NZ_CP086900:c225980-225762 [Pseudosulfitobacter pseudonitzschiae]
MPTSVSHRVQKRRDALRAAGLRPVQIWVPDTRRPGFEAECRRQAAIVAKADRADDTLNDFLDASLVDVDDWE

Nucleotide

• Length: 219 bp

>AT224560 NZ_CP120346:49898-50116 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGAAGCAGCGTATTACTGTCACCATAGACAGTGACAGCTATCAGTTACTCAAGTCCGCAGACGTGAATATCTCCGGCCT
GGTAAATACTGCCATGCAGAAGGAGGCCCGCCGTCTGCGTGCGGAACGCTGGCAGGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAGCGAACGGCTCCTTTGCAGACGAAAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A073IW87

Antitoxin

• AlphaFold DB: A0A073IWP4