Detailed information of TA system TA224558

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 90584..91196
• Replicon: plasmid P3
• Accession: NZ_CP086897
• Organism: Pseudosulfitobacter pseudonitzschiae strain SC1-37

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LOE20_RS21405
• Protein ID: WP_028288627.1
• Coordinates: 90584..90979 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A921TG38
• Locus tag: LOE20_RS21410
• Protein ID: WP_009503866.1
• Coordinates: 90969..91196 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE20_RS21385 (LOE20_21390)	87103..87714	-	612	WP_197919471.1	SMC-Scp complex subunit ScpB	-
LOE20_RS21390 (LOE20_21395)	87762..88646	-	885	WP_037239259.1	DUF1403 family protein	-
LOE20_RS21395 (LOE20_21400)	88761..89912	+	1152	WP_224557873.1	tyrosine-type recombinase/integrase	-
LOE20_RS21400 (LOE20_21405)	90014..90505	+	492	Protein_92	recombinase family protein	-
LOE20_RS21405 (LOE20_21410)	90584..90979	-	396	WP_028288627.1	PIN domain-containing protein	Toxin
LOE20_RS21410 (LOE20_21415)	90969..91196	-	228	WP_009503866.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LOE20_RS21415 (LOE20_21420)	91405..92598	+	1194	WP_009503867.1	plasmid partitioning protein RepA	-
LOE20_RS21420 (LOE20_21425)	92582..93514	+	933	WP_028288626.1	plasmid partitioning protein RepB	-
LOE20_RS21425 (LOE20_21430)	93728..94939	+	1212	WP_050525470.1	plasmid replication protein RepC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..110568	110568

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-113)

Antitoxin

• Antitoxin PemI-like (2-47)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14212.23 Da
• Isoelectric Point: 4.1653

>T224558 WP_028288627.1 NZ_CP086897:c90979-90584 [Pseudosulfitobacter pseudonitzschiae]
MSVEFADTNVVLYLLDDGPKADRAEVILGQGPRISVQVLNESLVNCRRKAGLGWEEAAAFLEGVRALCPVEDLTVQTHDV
GRALAERYGFSIYDAMIVASALVAGCTTLWSEDMQDGLLVEGQLRIVNPFA

Nucleotide

• Length: 396 bp

>T224558 NZ_CP120346:28887-29369 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGGAAATAAGTGTTACTGCACCGGAGTTGCTAAACGAGGAACACTATCTTCAACAGTTTGATTGCGGGAATGATGTGCT
CAGTGACTGGCTTCGCCGCAGAGCGATGAAGAACCAATACCTCAATGCCTCCCGTACATTTGTTATCTGCCCTGAAGGCA
CAAAGCGAGTAGTGGGATATTACTCTATTGCGACGGGGTCTGTAAGTCATGCCAGTTTAGGTCGCAGTTTACGCCAGAAT
ATGCCCGATCCTGTTCCGGTTGTTTTGCTGGGACGTCTTGCTGTTGATGAGTGTACTCAAGGGCATAGTTTTGGAAAATG
GCTGCTTAATGATGCTGTAACACGGGTATCTAACCTGGCAGACCAGGTGGGTATTAAAGCGATCATGGTTCATGCTATTG
ATGAACAGGCGAAAACATTCTATGAATACTTTGGCTTTGTTCAGTCTCCCATTGCGCCAAATACATTGTTCTACAAGATT
TAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8535.79 Da
• Isoelectric Point: 11.0482

>AT224558 WP_009503866.1 NZ_CP086897:c91196-90969 [Pseudosulfitobacter pseudonitzschiae]
MQVAKWGNSLAVRLPAELVRELGLKEGDQIDLVKDDGRVRVRRLARADEVLTGLRRFRGKLSAAERLSRDDAHER

Nucleotide

• Length: 228 bp

>AT224558 NZ_CP120346:28612-28896 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGCAAACTACAATCCGCAAATCTGTGCGTAATAAACAAATAAATATACGGGCTACTGATGAGGAACGTGCTGTGATTGA
TTATGCAGCGAGCCTGGTCAGTAAAAACCGTACCGACTTCATCATAGAAAAGGCTGTGAGCGAGGCCCAGAATATCATTC
TGGATCAACGCGTTTTTGTTCTGGATGATGCCCGCTATCAGGCTTTTATTAAACAACTTGAAGCTCCTGTACAGAATACT
GAAGGTCGTCAGCGCCTGATGGATGTGAAACCTGAATGGAAATAA