Detailed information of TA system TA224555

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 3812467..3812932
• Replicon: chromosome
• Accession: NZ_CP086894
• Organism: Pseudosulfitobacter pseudonitzschiae strain SC1-37

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LOE20_RS19045
• Protein ID: WP_224557762.1
• Coordinates: 3812467..3812730 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LOE20_RS19050
• Protein ID: WP_224557764.1
• Coordinates: 3812717..3812932 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE20_RS19020 (LOE20_19030)	3808183..3809733	-	1551	WP_224557015.1	restriction endonuclease subunit S	-
LOE20_RS19025 (LOE20_19035)	3809730..3810026	-	297	WP_224557153.1	type I restriction-modification enzyme R subunit C-terminal domain-containing protein	-
LOE20_RS19030 (LOE20_19040)	3809920..3810408	-	489	WP_229938596.1	DEAD/DEAH box helicase family protein	-
LOE20_RS19035 (LOE20_19045)	3810634..3811743	-	1110	WP_224558030.1	IS110 family transposase	-
LOE20_RS19040 (LOE20_19050)	3811836..3812279	-	444	WP_224557760.1	hypothetical protein	-
LOE20_RS19045 (LOE20_19055)	3812467..3812730	+	264	WP_224557762.1	BrnT family toxin	Toxin
LOE20_RS19050 (LOE20_19060)	3812717..3812932	+	216	WP_224557764.1	CopG family antitoxin	Antitoxin
LOE20_RS19055 (LOE20_19065)	3813285..3813509	-	225	WP_224557765.1	hypothetical protein	-
LOE20_RS19060 (LOE20_19070)	3813677..3814057	-	381	WP_224557766.1	helix-turn-helix domain-containing protein	-
LOE20_RS19065 (LOE20_19075)	3814238..3815209	-	972	WP_224557767.1	hypothetical protein	-
LOE20_RS19070 (LOE20_19080)	3815202..3816386	-	1185	WP_224557768.1	site-specific integrase	-
LOE20_RS19080 (LOE20_19090)	3816764..3817462	-	699	WP_037931342.1	SURF1 family protein	-
LOE20_RS19085 (LOE20_19095)	3817459..3817824	-	366	WP_028957811.1	cytochrome o ubiquinol oxidase subunit IV	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3804086..3816386	12300

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-80)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10254.52 Da
• Isoelectric Point: 5.6550

>T224555 WP_224557762.1 NZ_CP086894:3812467-3812730 [Pseudosulfitobacter pseudonitzschiae]
MKFEYDPDKSAGNKAKHGIDFDEAQALWEDPWLLEAPARTDDEPRFFAIGMIKERHWSAVCTYRGDIIRIISVRRARKQE
IEHYEST

Nucleotide

• Length: 264 bp

>T224555 NZ_CP120345:c4666496-4666185 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8265.40 Da
• Isoelectric Point: 10.6538

>AT224555 WP_224557764.1 NZ_CP086894:3812717-3812932 [Pseudosulfitobacter pseudonitzschiae]
MKAREFDQKFDTGENVSDLVDWSKARRTNTQTKRVNVDFPSWVVSGLDQKAKQLGVTRQSLIKLWIAERLQ

Nucleotide

• Length: 216 bp

>AT224555 NZ_CP120345:c4666184-4665894 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAACCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAGC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA