Detailed information of TA system TA224546

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 76811..77485
• Replicon: plasmid P7
• Accession: NZ_CP086884
• Organism: Pseudosulfitobacter pseudonitzschiae strain SC1-89

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LOE22_RS23345
• Protein ID: WP_224557924.1
• Coordinates: 77063..77485 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LOE22_RS23340
• Protein ID: WP_229939240.1
• Coordinates: 76811..77059 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE22_RS23320 (LOE22_23315)	72724..73368	+	645	WP_224557929.1	type IV secretion system protein	-
LOE22_RS23325 (LOE22_23320)	73372..74103	+	732	WP_224557928.1	TrbG/VirB9 family P-type conjugative transfer protein	-
LOE22_RS23330 (LOE22_23325)	74106..75563	+	1458	WP_224557927.1	TrbI/VirB10 family protein	-
LOE22_RS23335 (LOE22_23330)	75674..76663	+	990	WP_224557926.1	P-type DNA transfer ATPase VirB11	-
LOE22_RS23340 (LOE22_23335)	76811..77059	+	249	WP_229939240.1	plasmid stabilization protein	Antitoxin
LOE22_RS23345 (LOE22_23340)	77063..77485	+	423	WP_224557924.1	type II toxin-antitoxin system VapC family toxin	Toxin
LOE22_RS23350 (LOE22_23345)	77482..80232	+	2751	WP_224557923.1	lactate dehydrogenase	-
LOE22_RS23355 (LOE22_23350)	80229..81287	+	1059	WP_224557922.1	HEPN domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..157664	157664

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15255.26 Da
• Isoelectric Point: 4.3767

>T224546 WP_224557924.1 NZ_CP086884:77063-77485 [Pseudosulfitobacter pseudonitzschiae]
MIILDTNVICELLRPTPEPRIEQWLSAQDGLNVYLTSISEAELRYGLANMGNGKRRAALIDAVDRILREDLAGRILPFDS
DAAQAYASIAATRRDAGRPIAQADCQIASIAHACGATVATRNTPDFEGCEIDLINPWTTE

Nucleotide

• Length: 423 bp

>T224546 NZ_CP120344:50116-50421 [Salmonella enterica subsp. enterica serovar Paratyphi B]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTACCGCCTGTTTGTGGATGTGCAGAGCGACATCGTCGATAC
CCCTGGCCGGCGAATGGTGATCCCGCTGGCCAGTGCCCGTCTGCTGTCGGATAAGGTCTCCCGCGAACTTTATCCGGTGG
TGCATATCGGGGATGAGAGCTGGCGTCTGATGACCACGGATATGGCCAGTGTCCCGGCCTCATTCATCGGGGAAGAGGTG
GCCGAGCTCAGCCACAGGGAGAACGACATCAAAAACGCCATTAACCTGATGTTCTGGGGGATCTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9206.46 Da
• Isoelectric Point: 6.7281

>AT224546 WP_229939240.1 NZ_CP086884:76811-77059 [Pseudosulfitobacter pseudonitzschiae]
MASITIRNLDDDVKRRLRIRAAEHGRSMEEEAREILRQVVGDAKPNHDLAAAIRARVGPLGGVELDLPQREAMHEPPTFD
RA

Nucleotide

• Length: 249 bp

>AT224546 NZ_CP120344:49896-50114 [Salmonella enterica subsp. enterica serovar Paratyphi B]
ATGAAGCAGCGTATTACTGTCACCATAGACAGTGACAGCTATCAGTTACTCAAGTCCGCAGACGTGAATATCTCCGGCCT
GGTAAATACTGCCATGCAGAAGGAGGCCCGCCGTCTGCGTGCGGAACGCTGGCAGGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAGCGAACGGCTCCTTTGCAGACGAAAACAGGGACTGGTGA