Detailed information of TA system TA224541

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 37748..38213
• Replicon: chromosome
• Accession: NZ_CP086877
• Organism: Pseudosulfitobacter pseudonitzschiae strain SC1-89

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LOE22_RS00190
• Protein ID: WP_224557762.1
• Coordinates: 37950..38213 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LOE22_RS00185
• Protein ID: WP_224557764.1
• Coordinates: 37748..37963 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE22_RS00150 (LOE22_00150)	32856..33221	+	366	WP_028957811.1	cytochrome o ubiquinol oxidase subunit IV	-
LOE22_RS00155 (LOE22_00155)	33218..33916	+	699	WP_037931342.1	SURF1 family protein	-
LOE22_RS00165 (LOE22_00165)	34294..35478	+	1185	WP_224557768.1	site-specific integrase	-
LOE22_RS00170 (LOE22_00170)	35471..36442	+	972	WP_224557767.1	hypothetical protein	-
LOE22_RS00175 (LOE22_00175)	36623..37003	+	381	WP_224557766.1	helix-turn-helix domain-containing protein	-
LOE22_RS00180 (LOE22_00180)	37171..37395	+	225	WP_224557765.1	hypothetical protein	-
LOE22_RS00185 (LOE22_00185)	37748..37963	-	216	WP_224557764.1	CopG family antitoxin	Antitoxin
LOE22_RS00190 (LOE22_00190)	37950..38213	-	264	WP_224557762.1	BrnT family toxin	Toxin
LOE22_RS00195 (LOE22_00195)	38401..38844	+	444	WP_224557760.1	hypothetical protein	-
LOE22_RS00200 (LOE22_00200)	38937..40046	+	1110	WP_224558030.1	IS110 family transposase	-
LOE22_RS00205 (LOE22_00205)	40272..40760	+	489	WP_229938596.1	DEAD/DEAH box helicase family protein	-
LOE22_RS00210 (LOE22_00210)	40684..40950	+	267	WP_229938971.1	type I restriction-modification enzyme R subunit C-terminal domain-containing protein	-
LOE22_RS00215 (LOE22_00215)	40947..42497	+	1551	WP_224557015.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-80)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10254.52 Da
• Isoelectric Point: 5.6550

>T224541 WP_224557762.1 NZ_CP086877:c38213-37950 [Pseudosulfitobacter pseudonitzschiae]
MKFEYDPDKSAGNKAKHGIDFDEAQALWEDPWLLEAPARTDDEPRFFAIGMIKERHWSAVCTYRGDIIRIISVRRARKQE
IEHYEST

Nucleotide

• Length: 264 bp

>T224541 NZ_CP120343:c4666501-4666190 [Salmonella enterica subsp. enterica serovar Paratyphi B]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8265.40 Da
• Isoelectric Point: 10.6538

>AT224541 WP_224557764.1 NZ_CP086877:c37963-37748 [Pseudosulfitobacter pseudonitzschiae]
MKAREFDQKFDTGENVSDLVDWSKARRTNTQTKRVNVDFPSWVVSGLDQKAKQLGVTRQSLIKLWIAERLQ

Nucleotide

• Length: 216 bp

>AT224541 NZ_CP120343:c4666189-4665899 [Salmonella enterica subsp. enterica serovar Paratyphi B]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAACCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAGC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA