Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/PemK-MazE |
| Location | 202941..203482 | Replicon | plasmid P1 |
| Accession | NZ_CP086863 | ||
| Organism | Pseudosulfitobacter pseudonitzschiae strain SC33-17 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | A0A073IW87 |
| Locus tag | LOE29_RS20095 | Protein ID | WP_037931244.1 |
| Coordinates | 202941..203267 (-) | Length | 109 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | A0A073IWP4 |
| Locus tag | LOE29_RS20100 | Protein ID | WP_037931247.1 |
| Coordinates | 203264..203482 (-) | Length | 73 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LOE29_RS20065 (LOE29_20060) | 198235..198732 | - | 498 | WP_037931962.1 | DUF5519 family protein | - |
| LOE29_RS20070 (LOE29_20065) | 198741..199556 | - | 816 | WP_224556226.1 | class III extradiol ring-cleavage dioxygenase | - |
| LOE29_RS20075 (LOE29_20070) | 199620..200027 | - | 408 | WP_157802081.1 | DoxX family protein | - |
| LOE29_RS20080 (LOE29_20075) | 200190..200513 | + | 324 | WP_027238707.1 | helix-turn-helix domain-containing protein | - |
| LOE29_RS20085 (LOE29_20080) | 200849..202421 | + | 1573 | Protein_195 | IS21 family transposase | - |
| LOE29_RS20090 (LOE29_20085) | 202411..202767 | + | 357 | Protein_196 | ATP-binding protein | - |
| LOE29_RS20095 (LOE29_20090) | 202941..203267 | - | 327 | WP_037931244.1 | type II toxin-antitoxin system PemK/MazF family toxin | Toxin |
| LOE29_RS20100 (LOE29_20095) | 203264..203482 | - | 219 | WP_037931247.1 | antitoxin MazE family protein | Antitoxin |
| LOE29_RS20105 (LOE29_20100) | 203780..204913 | - | 1134 | WP_037931249.1 | alpha/beta fold hydrolase | - |
| LOE29_RS20110 (LOE29_20105) | 204917..205381 | - | 465 | WP_051694690.1 | MaoC/PaaZ C-terminal domain-containing protein | - |
| LOE29_RS20115 (LOE29_20110) | 205436..206983 | - | 1548 | WP_073194592.1 | AMP-binding protein | - |
| LOE29_RS20120 (LOE29_20115) | 207218..208225 | - | 1008 | WP_081855484.1 | PaaX family transcriptional regulator C-terminal domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..290598 | 290598 | |
| - | flank | IS/Tn | - | - | 201135..202421 | 1286 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 109 a.a. Molecular weight: 11610.50 Da Isoelectric Point: 8.8545
>T224531 WP_037931244.1 NZ_CP086863:c203267-202941 [Pseudosulfitobacter pseudonitzschiae]
VRRGELVTVALQGDHGKPRPALVIQSDRFPDTATTTVLLVTSTLVDAPLIRLTVEPSAENGLRMRSQIMIDKAVTVRTDK
LGKAFGQLDDTAMIEVNRALALFLGVAG
VRRGELVTVALQGDHGKPRPALVIQSDRFPDTATTTVLLVTSTLVDAPLIRLTVEPSAENGLRMRSQIMIDKAVTVRTDK
LGKAFGQLDDTAMIEVNRALALFLGVAG
Download Length: 327 bp
>T224531 NZ_CP120341:4489958-4490269 [Salmonella enterica subsp. enterica serovar Muenchen]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATGGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATATTATCGGGGTAATAAAGAATGA
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATGGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATATTATCGGGGTAATAAAGAATGA
Antitoxin
Download Length: 73 a.a. Molecular weight: 8175.17 Da Isoelectric Point: 5.8017
>AT224531 WP_037931247.1 NZ_CP086863:c203482-203264 [Pseudosulfitobacter pseudonitzschiae]
MPTSVSHRVQKRRDALRAAGLRPVQIWVPDTRRPGFEAECRRQAAIVAKADRADDTLNDFLDASLVDVDDWE
MPTSVSHRVQKRRDALRAAGLRPVQIWVPDTRRPGFEAECRRQAAIVAKADRADDTLNDFLDASLVDVDDWE
Download Length: 219 bp
>AT224531 NZ_CP120341:4490266-4490712 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAACCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAATCACTCAAC
CAACACCTGGAGAAAACACCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTCAAACTACCAGCAGATGCCTTCATCGAGTAG
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAACCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAATCACTCAAC
CAACACCTGGAGAAAACACCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTCAAACTACCAGCAGATGCCTTCATCGAGTAG
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A073IW87 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A073IWP4 |