Detailed information of TA system TA224528

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 1561538..1562003
• Replicon: chromosome
• Accession: NZ_CP086862
• Organism: Pseudosulfitobacter pseudonitzschiae strain SC33-17

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LOE29_RS07985
• Protein ID: WP_224557762.1
• Coordinates: 1561538..1561801 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LOE29_RS07990
• Protein ID: WP_224557764.1
• Coordinates: 1561788..1562003 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE29_RS07960 (LOE29_07960)	1557254..1558804	-	1551	WP_224557015.1	restriction endonuclease subunit S	-
LOE29_RS07965 (LOE29_07965)	1558801..1559097	-	297	WP_224557153.1	type I restriction-modification enzyme R subunit C-terminal domain-containing protein	-
LOE29_RS07970 (LOE29_07970)	1558991..1559479	-	489	WP_229938596.1	DEAD/DEAH box helicase family protein	-
LOE29_RS07975 (LOE29_07975)	1559705..1560814	-	1110	WP_224558030.1	IS110 family transposase	-
LOE29_RS07980 (LOE29_07980)	1560907..1561350	-	444	WP_224557760.1	hypothetical protein	-
LOE29_RS07985 (LOE29_07985)	1561538..1561801	+	264	WP_224557762.1	BrnT family toxin	Toxin
LOE29_RS07990 (LOE29_07990)	1561788..1562003	+	216	WP_224557764.1	CopG family antitoxin	Antitoxin
LOE29_RS07995 (LOE29_07995)	1562356..1562580	-	225	WP_224557765.1	hypothetical protein	-
LOE29_RS08000 (LOE29_08000)	1562748..1563128	-	381	WP_224557766.1	helix-turn-helix domain-containing protein	-
LOE29_RS08005 (LOE29_08005)	1563309..1564280	-	972	WP_224557767.1	hypothetical protein	-
LOE29_RS08010 (LOE29_08010)	1564273..1565457	-	1185	WP_224557768.1	site-specific integrase	-
LOE29_RS08020 (LOE29_08020)	1565835..1566533	-	699	WP_037931342.1	SURF1 family protein	-
LOE29_RS08025 (LOE29_08025)	1566530..1566895	-	366	WP_028957811.1	cytochrome o ubiquinol oxidase subunit IV	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-80)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10254.52 Da
• Isoelectric Point: 5.6550

>T224528 WP_224557762.1 NZ_CP086862:1561538-1561801 [Pseudosulfitobacter pseudonitzschiae]
MKFEYDPDKSAGNKAKHGIDFDEAQALWEDPWLLEAPARTDDEPRFFAIGMIKERHWSAVCTYRGDIIRIISVRRARKQE
IEHYEST

Nucleotide

• Length: 264 bp

>T224528 NZ_CP120341:3388406-3388624 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8265.40 Da
• Isoelectric Point: 10.6538

>AT224528 WP_224557764.1 NZ_CP086862:1561788-1562003 [Pseudosulfitobacter pseudonitzschiae]
MKAREFDQKFDTGENVSDLVDWSKARRTNTQTKRVNVDFPSWVVSGLDQKAKQLGVTRQSLIKLWIAERLQ

Nucleotide

• Length: 216 bp

>AT224528 NZ_CP120341:3388004-3388378 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA