Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/PemK-MazE |
| Location | 172715..173256 | Replicon | plasmid P2 |
| Accession | NZ_CP086856 | ||
| Organism | Pseudosulfitobacter pseudonitzschiae strain SC33-25 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | A0A073IW87 |
| Locus tag | LOE30_RS21320 | Protein ID | WP_037931244.1 |
| Coordinates | 172930..173256 (+) | Length | 109 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | A0A073IWP4 |
| Locus tag | LOE30_RS21315 | Protein ID | WP_037931247.1 |
| Coordinates | 172715..172933 (+) | Length | 73 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LOE30_RS21295 (LOE30_21290) | 167972..168979 | + | 1008 | WP_081855484.1 | PaaX family transcriptional regulator C-terminal domain-containing protein | - |
| LOE30_RS21300 (LOE30_21295) | 169214..170761 | + | 1548 | WP_073194592.1 | AMP-binding protein | - |
| LOE30_RS21305 (LOE30_21300) | 170816..171280 | + | 465 | WP_051694690.1 | MaoC/PaaZ C-terminal domain-containing protein | - |
| LOE30_RS21310 (LOE30_21305) | 171284..172417 | + | 1134 | WP_037931249.1 | alpha/beta fold hydrolase | - |
| LOE30_RS21315 (LOE30_21310) | 172715..172933 | + | 219 | WP_037931247.1 | antitoxin MazE family protein | Antitoxin |
| LOE30_RS21320 (LOE30_21315) | 172930..173256 | + | 327 | WP_037931244.1 | type II toxin-antitoxin system PemK/MazF family toxin | Toxin |
| LOE30_RS21325 (LOE30_21320) | 173430..173786 | - | 357 | Protein_165 | ATP-binding protein | - |
| LOE30_RS21330 (LOE30_21325) | 173776..175348 | - | 1573 | Protein_166 | IS21 family transposase | - |
| LOE30_RS21335 (LOE30_21330) | 175684..176007 | - | 324 | WP_027238707.1 | helix-turn-helix domain-containing protein | - |
| LOE30_RS21340 (LOE30_21335) | 176170..176577 | + | 408 | WP_157802081.1 | DoxX family protein | - |
| LOE30_RS21345 (LOE30_21340) | 176641..177456 | + | 816 | WP_224556226.1 | class III extradiol ring-cleavage dioxygenase | - |
| LOE30_RS21350 (LOE30_21345) | 177465..177962 | + | 498 | WP_037931962.1 | DUF5519 family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..290594 | 290594 | |
| - | flank | IS/Tn | - | - | 173776..175062 | 1286 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 109 a.a. Molecular weight: 11610.50 Da Isoelectric Point: 8.8545
>T224525 WP_037931244.1 NZ_CP086856:172930-173256 [Pseudosulfitobacter pseudonitzschiae]
VRRGELVTVALQGDHGKPRPALVIQSDRFPDTATTTVLLVTSTLVDAPLIRLTVEPSAENGLRMRSQIMIDKAVTVRTDK
LGKAFGQLDDTAMIEVNRALALFLGVAG
VRRGELVTVALQGDHGKPRPALVIQSDRFPDTATTTVLLVTSTLVDAPLIRLTVEPSAENGLRMRSQIMIDKAVTVRTDK
LGKAFGQLDDTAMIEVNRALALFLGVAG
Download Length: 327 bp
>T224525 NZ_CP120341:c990493-989966 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTATTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATACGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTGTGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGCGGCGTAGCCCTGCTTATTGATG
CTAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTATCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTATTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATACGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTGTGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGCGGCGTAGCCCTGCTTATTGATG
CTAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTATCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA
Antitoxin
Download Length: 73 a.a. Molecular weight: 8175.17 Da Isoelectric Point: 5.8017
>AT224525 WP_037931247.1 NZ_CP086856:172715-172933 [Pseudosulfitobacter pseudonitzschiae]
MPTSVSHRVQKRRDALRAAGLRPVQIWVPDTRRPGFEAECRRQAAIVAKADRADDTLNDFLDASLVDVDDWE
MPTSVSHRVQKRRDALRAAGLRPVQIWVPDTRRPGFEAECRRQAAIVAKADRADDTLNDFLDASLVDVDDWE
Download Length: 219 bp
>AT224525 NZ_CP120341:c990780-990490 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGGCTGTCGTTACGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGTGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCTGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGGCTGTCGTTACGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGTGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCTGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A073IW87 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A073IWP4 |