Detailed information of TA system TA224520

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 97213..97825
• Replicon: plasmid P5
• Accession: NZ_CP086852
• Organism: Pseudosulfitobacter pseudonitzschiae strain SC33-87

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LOE31_RS23810
• Protein ID: WP_028288627.1
• Coordinates: 97430..97825 (+)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A921TG38
• Locus tag: LOE31_RS23805
• Protein ID: WP_009503866.1
• Coordinates: 97213..97440 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE31_RS23790 (LOE31_23780)	93470..94681	-	1212	WP_050525470.1	plasmid replication protein RepC	-
LOE31_RS23795 (LOE31_23785)	94895..95827	-	933	WP_028288626.1	plasmid partitioning protein RepB	-
LOE31_RS23800 (LOE31_23790)	95811..97004	-	1194	WP_009503867.1	plasmid partitioning protein RepA	-
LOE31_RS23805 (LOE31_23795)	97213..97440	+	228	WP_009503866.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LOE31_RS23810 (LOE31_23800)	97430..97825	+	396	WP_028288627.1	PIN domain-containing protein	Toxin
LOE31_RS23815 (LOE31_23805)	97904..98395	-	492	Protein_103	recombinase family protein	-
LOE31_RS23820 (LOE31_23810)	98497..99648	-	1152	WP_224557873.1	tyrosine-type recombinase/integrase	-
LOE31_RS23825 (LOE31_23815)	99763..100647	+	885	WP_229996243.1	DUF1403 family protein	-
LOE31_RS23830 (LOE31_23820)	100695..101306	+	612	WP_197919471.1	SMC-Scp complex subunit ScpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..110568	110568

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-113)

Antitoxin

• Antitoxin PemI-like (2-47)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14212.23 Da
• Isoelectric Point: 4.1653

>T224520 WP_028288627.1 NZ_CP086852:97430-97825 [Pseudosulfitobacter pseudonitzschiae]
MSVEFADTNVVLYLLDDGPKADRAEVILGQGPRISVQVLNESLVNCRRKAGLGWEEAAAFLEGVRALCPVEDLTVQTHDV
GRALAERYGFSIYDAMIVASALVAGCTTLWSEDMQDGLLVEGQLRIVNPFA

Nucleotide

• Length: 396 bp

>T224520 NZ_CP120340:c49046-48630 [Salmonella enterica subsp. enterica serovar Muenchen]
GTGAAGAAAATGTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCCGAAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCACCGTATTGTGGTCTCGGCCATCACCTACTCAGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGTCACATTGAACTGGTTGACGCGTTCTGCGCCCGCCTCGATGCTATCCTGCCCTGGGACCGGGCC
GCGGTGAACGCCACCACGGAGATTAAGGTGGCGCTACGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCGATCGC
CGGGCACGCTATTGCCGCCGGCGCGGTACTGGTGACAAATAATACGCGGGAGTTTGAGCGGGTGCCGGGTCTTGTTCTGG
AAGACTGGGGAAGATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8535.79 Da
• Isoelectric Point: 11.0482

>AT224520 WP_009503866.1 NZ_CP086852:97213-97440 [Pseudosulfitobacter pseudonitzschiae]
MQVAKWGNSLAVRLPAELVRELGLKEGDQIDLVKDDGRVRVRRLARADEVLTGLRRFRGKLSAAERLSRDDAHER

Nucleotide

• Length: 228 bp

>AT224520 NZ_CP120340:c49273-49043 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGAGAACCGTATCTATTTTTAAAAACGGCAACAACCGCGCCATCCGCCTTCCCCGCGATCTGGATTTTGATGGAGTGAG
CGAGCTGGAAATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCTGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTCG
AAAAAGCTGACCCGGGCTTTATGGCGGAGCGTGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA