Detailed information of TA system TA224518

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 224674..225215
• Replicon: plasmid P1
• Accession: NZ_CP086848
• Organism: Pseudosulfitobacter pseudonitzschiae strain SC33-87

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A073IW87
• Locus tag: LOE31_RS20215
• Protein ID: WP_037931244.1
• Coordinates: 224889..225215 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A073IWP4
• Locus tag: LOE31_RS20210
• Protein ID: WP_037931247.1
• Coordinates: 224674..224892 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE31_RS20190 (LOE31_20185)	219931..220938	+	1008	WP_081855484.1	PaaX family transcriptional regulator C-terminal domain-containing protein	-
LOE31_RS20195 (LOE31_20190)	221173..222720	+	1548	WP_073194592.1	AMP-binding protein	-
LOE31_RS20200 (LOE31_20195)	222775..223239	+	465	WP_051694690.1	MaoC/PaaZ C-terminal domain-containing protein	-
LOE31_RS20205 (LOE31_20200)	223243..224376	+	1134	WP_037931249.1	alpha/beta fold hydrolase	-
LOE31_RS20210 (LOE31_20205)	224674..224892	+	219	WP_037931247.1	antitoxin MazE family protein	Antitoxin
LOE31_RS20215 (LOE31_20210)	224889..225215	+	327	WP_037931244.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LOE31_RS20220 (LOE31_20215)	225389..225745	-	357	Protein_216	ATP-binding protein	-
LOE31_RS20225 (LOE31_20220)	225735..227307	-	1573	Protein_217	IS21 family transposase	-
LOE31_RS20230 (LOE31_20225)	227643..227966	-	324	WP_027238707.1	helix-turn-helix domain-containing protein	-
LOE31_RS20235 (LOE31_20230)	228129..228536	+	408	WP_229996238.1	DoxX family protein	-
LOE31_RS20240 (LOE31_20235)	228600..229415	+	816	WP_224556226.1	class III extradiol ring-cleavage dioxygenase	-
LOE31_RS20245 (LOE31_20240)	229424..229921	+	498	WP_037931962.1	DUF5519 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..290594	290594
	-	flank	IS/Tn	-	-	225735..227021	1286

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-105)

Antitoxin

• Antitoxin MazE-like (7-71)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11610.50 Da
• Isoelectric Point: 8.8545

>T224518 WP_037931244.1 NZ_CP086848:224889-225215 [Pseudosulfitobacter pseudonitzschiae]
VRRGELVTVALQGDHGKPRPALVIQSDRFPDTATTTVLLVTSTLVDAPLIRLTVEPSAENGLRMRSQIMIDKAVTVRTDK
LGKAFGQLDDTAMIEVNRALALFLGVAG

Nucleotide

• Length: 327 bp

>T224518 NZ_CP120340:14789-15121 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGGACAGAGGGGAAATCTGGCTCGTCTCACTGGATCCTACAGCCGGACATGAACAAAGCGGAAAACGTCCGGTACTTAT
CGTTTCTCCGGCATCATTTAATAAGTTCACCCGGTTACCCGTTGTCGTTCCCGTCACCAGCGGCGGAGACTTTGCCCGCA
CAGCCGGTTTTACCGTCTCCCTGGACGGCGCGGGAACAAAAACAACGGGTGTTATTCGCTGCGACCAGCCCAGAACGATT
GATATGGGTGCCCGGAATGGTAAGCGTCTGGAACGTATACCCGAAGCTGTCGTAAATGAAGTGCTGGCCCGACTGGAAGC
CATTCTGAGCTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8175.17 Da
• Isoelectric Point: 5.8017

>AT224518 WP_037931247.1 NZ_CP086848:224674-224892 [Pseudosulfitobacter pseudonitzschiae]
MPTSVSHRVQKRRDALRAAGLRPVQIWVPDTRRPGFEAECRRQAAIVAKADRADDTLNDFLDASLVDVDDWE

Nucleotide

• Length: 219 bp

>AT224518 NZ_CP120340:14531-14788 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGTATACCACTCGTCTGAAAAAGGTTGGCGGATCCGTCATGCTGGCGGTTCCTCCCGCCGTGTTGAAAACGCTGGCTCT
GTCGACAGACAGCGAAGTGGGAATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAACTGCTGGCACAGTGCGATCCGCACGCCGAAATGAGCGATGAGGATAGGGAATGGATGGATGCGCCTGCAGTG
GGTAAGGAGATCCTGTAA

Structures

Toxin

• AlphaFold DB: A0A073IW87

Antitoxin

• AlphaFold DB: A0A073IWP4