Detailed information of TA system TA224510

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 2250654..2251119
• Replicon: chromosome
• Accession: NZ_CP086839
• Organism: Pseudosulfitobacter pseudonitzschiae strain TC12-31

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LOE24_RS11110
• Protein ID: WP_224557762.1
• Coordinates: 2250856..2251119 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LOE24_RS11105
• Protein ID: WP_224557764.1
• Coordinates: 2250654..2250869 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE24_RS11070 (LOE24_11040)	2245762..2246127	+	366	WP_028957811.1	cytochrome o ubiquinol oxidase subunit IV	-
LOE24_RS11075 (LOE24_11045)	2246124..2246822	+	699	WP_037931342.1	SURF1 family protein	-
LOE24_RS11085 (LOE24_11055)	2247200..2248384	+	1185	WP_224557768.1	site-specific integrase	-
LOE24_RS11090 (LOE24_11060)	2248377..2249348	+	972	WP_224557767.1	hypothetical protein	-
LOE24_RS11095 (LOE24_11065)	2249529..2249909	+	381	WP_224557766.1	helix-turn-helix domain-containing protein	-
LOE24_RS11100 (LOE24_11070)	2250077..2250301	+	225	WP_224557765.1	hypothetical protein	-
LOE24_RS11105 (LOE24_11075)	2250654..2250869	-	216	WP_224557764.1	CopG family antitoxin	Antitoxin
LOE24_RS11110 (LOE24_11080)	2250856..2251119	-	264	WP_224557762.1	BrnT family toxin	Toxin
LOE24_RS11115 (LOE24_11085)	2251307..2251750	+	444	WP_224557760.1	hypothetical protein	-
LOE24_RS11120 (LOE24_11090)	2251843..2252952	+	1110	WP_224558030.1	IS110 family transposase	-
LOE24_RS11125 (LOE24_11095)	2253178..2253666	+	489	WP_229938596.1	DEAD/DEAH box helicase family protein	-
LOE24_RS11130 (LOE24_11100)	2253590..2253856	+	267	WP_229938971.1	type I restriction-modification enzyme R subunit C-terminal domain-containing protein	-
LOE24_RS11135 (LOE24_11105)	2253853..2255403	+	1551	WP_224557015.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2248554..2259713	11159

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-80)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10254.52 Da
• Isoelectric Point: 5.6550

>T224510 WP_224557762.1 NZ_CP086839:c2251119-2250856 [Pseudosulfitobacter pseudonitzschiae]
MKFEYDPDKSAGNKAKHGIDFDEAQALWEDPWLLEAPARTDDEPRFFAIGMIKERHWSAVCTYRGDIIRIISVRRARKQE
IEHYEST

Nucleotide

• Length: 264 bp

>T224510 NZ_CP120339:854964-855377 [Salmonella enterica subsp. enterica serovar Muenchen]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTAATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGTTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8265.40 Da
• Isoelectric Point: 10.6538

>AT224510 WP_224557764.1 NZ_CP086839:c2250869-2250654 [Pseudosulfitobacter pseudonitzschiae]
MKAREFDQKFDTGENVSDLVDWSKARRTNTQTKRVNVDFPSWVVSGLDQKAKQLGVTRQSLIKLWIAERLQ

Nucleotide

• Length: 216 bp

>AT224510 NZ_CP120339:854717-854983 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA