Detailed information of TA system TA224507

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 131363..132037
• Replicon: plasmid P4
• Accession: NZ_CP086836
• Organism: Pseudosulfitobacter pseudonitzschiae strain TC12-33

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LOE25_RS22445
• Protein ID: WP_224557924.1
• Coordinates: 131363..131785 (-)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LOE25_RS22450
• Protein ID: WP_229939240.1
• Coordinates: 131789..132037 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE25_RS22435 (LOE25_22395)	127561..128619	-	1059	WP_224557922.1	HEPN domain-containing protein	-
LOE25_RS22440 (LOE25_22400)	128616..131366	-	2751	WP_224557923.1	lactate dehydrogenase	-
LOE25_RS22445 (LOE25_22405)	131363..131785	-	423	WP_224557924.1	type II toxin-antitoxin system VapC family toxin	Toxin
LOE25_RS22450 (LOE25_22410)	131789..132037	-	249	WP_229939240.1	plasmid stabilization protein	Antitoxin
LOE25_RS22455 (LOE25_22415)	132185..133174	-	990	WP_224557926.1	P-type DNA transfer ATPase VirB11	-
LOE25_RS22460 (LOE25_22420)	133285..134742	-	1458	WP_224557927.1	TrbI/VirB10 family protein	-
LOE25_RS22465 (LOE25_22425)	134745..135476	-	732	WP_224557928.1	TrbG/VirB9 family P-type conjugative transfer protein	-
LOE25_RS22470 (LOE25_22430)	135480..136124	-	645	WP_224557929.1	type IV secretion system protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..290592	290592

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15255.26 Da
• Isoelectric Point: 4.3767

>T224507 WP_224557924.1 NZ_CP086836:c131785-131363 [Pseudosulfitobacter pseudonitzschiae]
MIILDTNVICELLRPTPEPRIEQWLSAQDGLNVYLTSISEAELRYGLANMGNGKRRAALIDAVDRILREDLAGRILPFDS
DAAQAYASIAATRRDAGRPIAQADCQIASIAHACGATVATRNTPDFEGCEIDLINPWTTE

Nucleotide

• Length: 423 bp

>T224507 NZ_CP120338:c49047-48631 [Salmonella enterica subsp. enterica serovar Muenchen]
GTGAAGAAAATGTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCCGAAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCACCGTATTGTGGTCTCGGCCATCACCTACTCAGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGTCACATTGAACTGGTTGACGCGTTCTGCGCCCGCCTCGATGCTATCCTGCCCTGGGACCGGGCC
GCGGTGAACGCCACCACGGAGATTAAGGTGGCGCTACGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCGATCGC
CGGGCACGCTATTGCCGCCGGCGCGGTACTGGTGACAAATAATACGCGGGAGTTTGAGCGGGTGCCGGGTCTTGTTCTGG
AAGACTGGGGAAGATAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9206.46 Da
• Isoelectric Point: 6.7281

>AT224507 WP_229939240.1 NZ_CP086836:c132037-131789 [Pseudosulfitobacter pseudonitzschiae]
MASITIRNLDDDVKRRLRIRAAEHGRSMEEEAREILRQVVGDAKPNHDLAAAIRARVGPLGGVELDLPQREAMHEPPTFD
RA

Nucleotide

• Length: 249 bp

>AT224507 NZ_CP120338:c49274-49044 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGAGAACCGTATCTATTTTTAAAAACGGCAACAACCGCGCCATCCGCCTTCCCCGCGATCTGGATTTTGATGGAGTGAG
CGAGCTGGAAATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCTGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTCG
AAAAAGCTGACCCGGGCTTTATGGCGGAGCGTGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA