Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/PemK-MazE |
| Location | 26375..26916 | Replicon | plasmid P4 |
| Accession | NZ_CP086836 | ||
| Organism | Pseudosulfitobacter pseudonitzschiae strain TC12-33 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | A0A073IW87 |
| Locus tag | LOE25_RS21920 | Protein ID | WP_037931244.1 |
| Coordinates | 26590..26916 (+) | Length | 109 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | A0A073IWP4 |
| Locus tag | LOE25_RS21915 | Protein ID | WP_037931247.1 |
| Coordinates | 26375..26593 (+) | Length | 73 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LOE25_RS21895 (LOE25_21850) | 21632..22639 | + | 1008 | WP_081855484.1 | PaaX family transcriptional regulator C-terminal domain-containing protein | - |
| LOE25_RS21900 (LOE25_21855) | 22874..24421 | + | 1548 | WP_073194592.1 | AMP-binding protein | - |
| LOE25_RS21905 (LOE25_21860) | 24476..24940 | + | 465 | WP_051694690.1 | MaoC/PaaZ C-terminal domain-containing protein | - |
| LOE25_RS21910 (LOE25_21865) | 24944..26077 | + | 1134 | WP_037931249.1 | alpha/beta fold hydrolase | - |
| LOE25_RS21915 (LOE25_21870) | 26375..26593 | + | 219 | WP_037931247.1 | antitoxin MazE family protein | Antitoxin |
| LOE25_RS21920 (LOE25_21875) | 26590..26916 | + | 327 | WP_037931244.1 | type II toxin-antitoxin system PemK/MazF family toxin | Toxin |
| LOE25_RS21925 (LOE25_21880) | 27090..27446 | - | 357 | Protein_31 | ATP-binding protein | - |
| LOE25_RS21930 (LOE25_21885) | 27436..29008 | - | 1573 | Protein_32 | IS21 family transposase | - |
| LOE25_RS21935 (LOE25_21890) | 29344..29667 | - | 324 | WP_027238707.1 | helix-turn-helix domain-containing protein | - |
| LOE25_RS21940 (LOE25_21895) | 29830..30237 | + | 408 | WP_157802081.1 | DoxX family protein | - |
| LOE25_RS21945 (LOE25_21900) | 30301..31116 | + | 816 | WP_224556226.1 | class III extradiol ring-cleavage dioxygenase | - |
| LOE25_RS21950 (LOE25_21905) | 31125..31622 | + | 498 | WP_037931962.1 | DUF5519 family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..290592 | 290592 | |
| - | flank | IS/Tn | - | - | 27436..28722 | 1286 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 109 a.a. Molecular weight: 11610.50 Da Isoelectric Point: 8.8545
>T224506 WP_037931244.1 NZ_CP086836:26590-26916 [Pseudosulfitobacter pseudonitzschiae]
VRRGELVTVALQGDHGKPRPALVIQSDRFPDTATTTVLLVTSTLVDAPLIRLTVEPSAENGLRMRSQIMIDKAVTVRTDK
LGKAFGQLDDTAMIEVNRALALFLGVAG
VRRGELVTVALQGDHGKPRPALVIQSDRFPDTATTTVLLVTSTLVDAPLIRLTVEPSAENGLRMRSQIMIDKAVTVRTDK
LGKAFGQLDDTAMIEVNRALALFLGVAG
Download Length: 327 bp
>T224506 NZ_CP120338:28887-29369 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGGAAATAAGTGTTACTGCACCGGAGTTGCTAAACGAGGAACACTATCTTCAACAGTTTGATTGCGGGAATGATGTGCT
CAGTGACTGGCTTCGCCGCAGAGCGATGAAGAACCAATACCTCAATGCCTCCCGTACATTTGTTATCTGCCCTGAAGGCA
CAAAGCGAGTAGTGGGATATTACTCTATTGCGACGGGGTCTGTAAGTCATGCCAGTTTAGGTCGCAGTTTACGCCAGAAT
ATGCCCGATCCTGTTCCGGTTGTTTTGCTGGGACGTCTTGCTGTTGATGAGTGTACTCAAGGGCATAGTTTTGGAAAATG
GCTGCTTAATGATGCTGTAACACGGGTATCTAACCTGGCAGACCAGGTGGGTATTAAAGCGATCATGGTTCATGCTATTG
ATGAACAGGCGAAAACATTCTATGAATACTTTGGCTTTGTTCAGTCTCCCATTGCGCCAAATACATTGTTCTACAAGATT
TAA
ATGGAAATAAGTGTTACTGCACCGGAGTTGCTAAACGAGGAACACTATCTTCAACAGTTTGATTGCGGGAATGATGTGCT
CAGTGACTGGCTTCGCCGCAGAGCGATGAAGAACCAATACCTCAATGCCTCCCGTACATTTGTTATCTGCCCTGAAGGCA
CAAAGCGAGTAGTGGGATATTACTCTATTGCGACGGGGTCTGTAAGTCATGCCAGTTTAGGTCGCAGTTTACGCCAGAAT
ATGCCCGATCCTGTTCCGGTTGTTTTGCTGGGACGTCTTGCTGTTGATGAGTGTACTCAAGGGCATAGTTTTGGAAAATG
GCTGCTTAATGATGCTGTAACACGGGTATCTAACCTGGCAGACCAGGTGGGTATTAAAGCGATCATGGTTCATGCTATTG
ATGAACAGGCGAAAACATTCTATGAATACTTTGGCTTTGTTCAGTCTCCCATTGCGCCAAATACATTGTTCTACAAGATT
TAA
Antitoxin
Download Length: 73 a.a. Molecular weight: 8175.17 Da Isoelectric Point: 5.8017
>AT224506 WP_037931247.1 NZ_CP086836:26375-26593 [Pseudosulfitobacter pseudonitzschiae]
MPTSVSHRVQKRRDALRAAGLRPVQIWVPDTRRPGFEAECRRQAAIVAKADRADDTLNDFLDASLVDVDDWE
MPTSVSHRVQKRRDALRAAGLRPVQIWVPDTRRPGFEAECRRQAAIVAKADRADDTLNDFLDASLVDVDDWE
Download Length: 219 bp
>AT224506 NZ_CP120338:28612-28896 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGCAAACTACAATCCGCAAATCTGTGCGTAATAAACAAATAAATATACGGGCTACTGATGAGGAACGTGCTGTGATTGA
TTATGCAGCGAGCCTGGTCAGTAAAAACCGTACCGACTTCATCATAGAAAAGGCTGTGAGCGAGGCCCAGAATATCATTC
TGGATCAACGCGTTTTTGTTCTGGATGATGCCCGCTATCAGGCTTTTATTAAACAACTTGAAGCTCCTGTACAGAATACT
GAAGGTCGTCAGCGCCTGATGGATGTGAAACCTGAATGGAAATAA
ATGCAAACTACAATCCGCAAATCTGTGCGTAATAAACAAATAAATATACGGGCTACTGATGAGGAACGTGCTGTGATTGA
TTATGCAGCGAGCCTGGTCAGTAAAAACCGTACCGACTTCATCATAGAAAAGGCTGTGAGCGAGGCCCAGAATATCATTC
TGGATCAACGCGTTTTTGTTCTGGATGATGCCCGCTATCAGGCTTTTATTAAACAACTTGAAGCTCCTGTACAGAATACT
GAAGGTCGTCAGCGCCTGATGGATGTGAAACCTGAATGGAAATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A073IW87 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A073IWP4 |