Detailed information of TA system TA224499

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 14176..14850
• Replicon: plasmid P2
• Accession: NZ_CP086826
• Organism: Pseudosulfitobacter pseudonitzschiae strain TC12-5

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LOE28_RS19875
• Protein ID: WP_224557924.1
• Coordinates: 14428..14850 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LOE28_RS19870
• Protein ID: WP_229939240.1
• Coordinates: 14176..14424 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE28_RS19850 (LOE28_19855)	10089..10733	+	645	WP_224557929.1	type IV secretion system protein	-
LOE28_RS19855 (LOE28_19860)	10737..11468	+	732	WP_224557928.1	TrbG/VirB9 family P-type conjugative transfer protein	-
LOE28_RS19860 (LOE28_19865)	11471..12928	+	1458	WP_224557927.1	TrbI/VirB10 family protein	-
LOE28_RS19865 (LOE28_19870)	13039..14028	+	990	WP_224557926.1	P-type DNA transfer ATPase VirB11	-
LOE28_RS19870 (LOE28_19875)	14176..14424	+	249	WP_229939240.1	plasmid stabilization protein	Antitoxin
LOE28_RS19875 (LOE28_19880)	14428..14850	+	423	WP_224557924.1	type II toxin-antitoxin system VapC family toxin	Toxin
LOE28_RS19880 (LOE28_19885)	14847..17597	+	2751	WP_224557923.1	lactate dehydrogenase	-
LOE28_RS19885 (LOE28_19890)	17594..18652	+	1059	WP_224557922.1	HEPN domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..290592	290592

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15255.26 Da
• Isoelectric Point: 4.3767

>T224499 WP_224557924.1 NZ_CP086826:14428-14850 [Pseudosulfitobacter pseudonitzschiae]
MIILDTNVICELLRPTPEPRIEQWLSAQDGLNVYLTSISEAELRYGLANMGNGKRRAALIDAVDRILREDLAGRILPFDS
DAAQAYASIAATRRDAGRPIAQADCQIASIAHACGATVATRNTPDFEGCEIDLINPWTTE

Nucleotide

• Length: 423 bp

>T224499 NZ_CP120337:3522075-3522293 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9206.46 Da
• Isoelectric Point: 6.7281

>AT224499 WP_229939240.1 NZ_CP086826:14176-14424 [Pseudosulfitobacter pseudonitzschiae]
MASITIRNLDDDVKRRLRIRAAEHGRSMEEEAREILRQVVGDAKPNHDLAAAIRARVGPLGGVELDLPQREAMHEPPTFD
RA

Nucleotide

• Length: 249 bp

>AT224499 NZ_CP120337:3521673-3522047 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA