Detailed information of TA system TA224494

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 119302..119843
• Replicon: plasmid P2
• Accession: NZ_CP086818
• Organism: Pseudosulfitobacter pseudonitzschiae strain TC12-52

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A073IW87
• Locus tag: LOE26_RS20410
• Protein ID: WP_037931244.1
• Coordinates: 119302..119628 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A073IWP4
• Locus tag: LOE26_RS20415
• Protein ID: WP_037931247.1
• Coordinates: 119625..119843 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE26_RS20380 (LOE26_20375)	114596..115093	-	498	WP_037931962.1	DUF5519 family protein	-
LOE26_RS20385 (LOE26_20380)	115102..115917	-	816	WP_224556226.1	class III extradiol ring-cleavage dioxygenase	-
LOE26_RS20390 (LOE26_20385)	115981..116388	-	408	WP_157802081.1	DoxX family protein	-
LOE26_RS20395 (LOE26_20390)	116551..116874	+	324	WP_027238707.1	helix-turn-helix domain-containing protein	-
LOE26_RS20400 (LOE26_20395)	117210..118782	+	1573	Protein_120	IS21 family transposase	-
LOE26_RS20405 (LOE26_20400)	118772..119128	+	357	Protein_121	ATP-binding protein	-
LOE26_RS20410 (LOE26_20405)	119302..119628	-	327	WP_037931244.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LOE26_RS20415 (LOE26_20410)	119625..119843	-	219	WP_037931247.1	antitoxin MazE family protein	Antitoxin
LOE26_RS20420 (LOE26_20415)	120141..121274	-	1134	WP_037931249.1	alpha/beta fold hydrolase	-
LOE26_RS20425 (LOE26_20420)	121278..121742	-	465	WP_051694690.1	MaoC/PaaZ C-terminal domain-containing protein	-
LOE26_RS20430 (LOE26_20425)	121797..123344	-	1548	WP_073194592.1	AMP-binding protein	-
LOE26_RS20435 (LOE26_20430)	123579..124586	-	1008	WP_081855484.1	PaaX family transcriptional regulator C-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..290597	290597
	-	flank	IS/Tn	-	-	117496..118782	1286

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-105)

Antitoxin

• Antitoxin MazE-like (7-71)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11610.50 Da
• Isoelectric Point: 8.8545

>T224494 WP_037931244.1 NZ_CP086818:c119628-119302 [Pseudosulfitobacter pseudonitzschiae]
VRRGELVTVALQGDHGKPRPALVIQSDRFPDTATTTVLLVTSTLVDAPLIRLTVEPSAENGLRMRSQIMIDKAVTVRTDK
LGKAFGQLDDTAMIEVNRALALFLGVAG

Nucleotide

• Length: 327 bp

>T224494 NZ_CP120336:50118-50423 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTACCGCCTGTTTGTGGATGTGCAGAGCGACATCGTCGATAC
CCCTGGCCGGCGAATGGTGATCCCGCTGGCCAGTGCCCGTCTGCTGTCGGATAAGGTCTCCCGCGAACTTTATCCGGTGG
TGCATATCGGGGATGAGAGCTGGCGTCTGATGACCACGGATATGGCCAGTGTCCCGGCCTCATTCATCGGGGAAGAGGTG
GCCGAGCTCAGCCACAGGGAGAACGACATCAAAAACGCCATTAACCTGATGTTCTGGGGGATCTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8175.17 Da
• Isoelectric Point: 5.8017

>AT224494 WP_037931247.1 NZ_CP086818:c119843-119625 [Pseudosulfitobacter pseudonitzschiae]
MPTSVSHRVQKRRDALRAAGLRPVQIWVPDTRRPGFEAECRRQAAIVAKADRADDTLNDFLDASLVDVDDWE

Nucleotide

• Length: 219 bp

>AT224494 NZ_CP120336:49898-50116 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGAAGCAGCGTATTACTGTCACCATAGACAGTGACAGCTATCAGTTACTCAAGTCCGCAGACGTGAATATCTCCGGCCT
GGTAAATACTGCCATGCAGAAGGAGGCCCGCCGTCTGCGTGCGGAACGCTGGCAGGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAGCGAACGGCTCCTTTGCAGACGAAAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A073IW87

Antitoxin

• AlphaFold DB: A0A073IWP4