Detailed information of TA system TA224493

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 14179..14853
• Replicon: plasmid P2
• Accession: NZ_CP086818
• Organism: Pseudosulfitobacter pseudonitzschiae strain TC12-52

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LOE26_RS19880
• Protein ID: WP_224557924.1
• Coordinates: 14431..14853 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LOE26_RS19875
• Protein ID: WP_229939240.1
• Coordinates: 14179..14427 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE26_RS19855 (LOE26_19850)	10092..10736	+	645	WP_224557929.1	type IV secretion system protein	-
LOE26_RS19860 (LOE26_19855)	10740..11471	+	732	WP_224557928.1	TrbG/VirB9 family P-type conjugative transfer protein	-
LOE26_RS19865 (LOE26_19860)	11474..12931	+	1458	WP_224557927.1	TrbI/VirB10 family protein	-
LOE26_RS19870 (LOE26_19865)	13042..14031	+	990	WP_224557926.1	P-type DNA transfer ATPase VirB11	-
LOE26_RS19875 (LOE26_19870)	14179..14427	+	249	WP_229939240.1	plasmid stabilization protein	Antitoxin
LOE26_RS19880 (LOE26_19875)	14431..14853	+	423	WP_224557924.1	type II toxin-antitoxin system VapC family toxin	Toxin
LOE26_RS19885 (LOE26_19880)	14850..17600	+	2751	WP_224557923.1	lactate dehydrogenase	-
LOE26_RS19890 (LOE26_19885)	17597..18655	+	1059	WP_224557922.1	HEPN domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..290597	290597

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15255.26 Da
• Isoelectric Point: 4.3767

>T224493 WP_224557924.1 NZ_CP086818:14431-14853 [Pseudosulfitobacter pseudonitzschiae]
MIILDTNVICELLRPTPEPRIEQWLSAQDGLNVYLTSISEAELRYGLANMGNGKRRAALIDAVDRILREDLAGRILPFDS
DAAQAYASIAATRRDAGRPIAQADCQIASIAHACGATVATRNTPDFEGCEIDLINPWTTE

Nucleotide

• Length: 423 bp

>T224493 NZ_CP120336:c49047-48631 [Salmonella enterica subsp. enterica serovar Muenchen]
GTGAAGAAAATGTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCCGAAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCACCGTATTGTGGTCTCGGCCATCACCTACTCAGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGTCACATTGAACTGGTTGACGCGTTCTGCGCCCGCCTCGATGCTATCCTGCCCTGGGACCGGGCC
GCGGTGAACGCCACCACGGAGATTAAGGTGGCGCTACGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCGATCGC
CGGGCACGCTATTGCCGCCGGCGCGGTACTGGTGACAAATAATACGCGGGAGTTTGAGCGGGTGCCGGGTCTTGTTCTGG
AAGACTGGGGAAGATAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9206.46 Da
• Isoelectric Point: 6.7281

>AT224493 WP_229939240.1 NZ_CP086818:14179-14427 [Pseudosulfitobacter pseudonitzschiae]
MASITIRNLDDDVKRRLRIRAAEHGRSMEEEAREILRQVVGDAKPNHDLAAAIRARVGPLGGVELDLPQREAMHEPPTFD
RA

Nucleotide

• Length: 249 bp

>AT224493 NZ_CP120336:c49274-49044 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGAGAACCGTATCTATTTTTAAAAACGGCAACAACCGCGCCATCCGCCTTCCCCGCGATCTGGATTTTGATGGAGTGAG
CGAGCTGGAAATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCTGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTCG
AAAAAGCTGACCCGGGCTTTATGGCGGAGCGTGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA