Detailed information of TA system TA224492

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 3268..3880
• Replicon: plasmid P1
• Accession: NZ_CP086817
• Organism: Pseudosulfitobacter pseudonitzschiae strain TC12-52

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LOE26_RS19250
• Protein ID: WP_028288627.1
• Coordinates: 3268..3663 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A921TG38
• Locus tag: LOE26_RS19255
• Protein ID: WP_009503866.1
• Coordinates: 3653..3880 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE26_RS19235 (LOE26_19230)	446..1330	-	885	WP_037239259.1	DUF1403 family protein	-
LOE26_RS19240 (LOE26_19235)	1445..2596	+	1152	WP_224557873.1	tyrosine-type recombinase/integrase	-
LOE26_RS19245 (LOE26_19240)	2698..3189	+	492	Protein_3	recombinase family protein	-
LOE26_RS19250 (LOE26_19245)	3268..3663	-	396	WP_028288627.1	PIN domain-containing protein	Toxin
LOE26_RS19255 (LOE26_19250)	3653..3880	-	228	WP_009503866.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LOE26_RS19260 (LOE26_19255)	4089..5282	+	1194	WP_009503867.1	plasmid partitioning protein RepA	-
LOE26_RS19265 (LOE26_19260)	5266..6198	+	933	WP_028288626.1	plasmid partitioning protein RepB	-
LOE26_RS19270 (LOE26_19265)	6412..7623	+	1212	WP_050525470.1	plasmid replication protein RepC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..110566	110566

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-113)

Antitoxin

• Antitoxin PemI-like (2-47)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14212.23 Da
• Isoelectric Point: 4.1653

>T224492 WP_028288627.1 NZ_CP086817:c3663-3268 [Pseudosulfitobacter pseudonitzschiae]
MSVEFADTNVVLYLLDDGPKADRAEVILGQGPRISVQVLNESLVNCRRKAGLGWEEAAAFLEGVRALCPVEDLTVQTHDV
GRALAERYGFSIYDAMIVASALVAGCTTLWSEDMQDGLLVEGQLRIVNPFA

Nucleotide

• Length: 396 bp

>T224492 NZ_CP120336:28887-29369 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGGAAATAAGTGTTACTGCACCGGAGTTGCTAAACGAGGAACACTATCTTCAACAGTTTGATTGCGGGAATGATGTGCT
CAGTGACTGGCTTCGCCGCAGAGCGATGAAGAACCAATACCTCAATGCCTCCCGTACATTTGTTATCTGCCCTGAAGGCA
CAAAGCGAGTAGTGGGATATTACTCTATTGCGACGGGGTCTGTAAGTCATGCCAGTTTAGGTCGCAGTTTACGCCAGAAT
ATGCCCGATCCTGTTCCGGTTGTTTTGCTGGGACGTCTTGCTGTTGATGAGTGTACTCAAGGGCATAGTTTTGGAAAATG
GCTGCTTAATGATGCTGTAACACGGGTATCTAACCTGGCAGACCAGGTGGGTATTAAAGCGATCATGGTTCATGCTATTG
ATGAACAGGCGAAAACATTCTATGAATACTTTGGCTTTGTTCAGTCTCCCATTGCGCCAAATACATTGTTCTACAAGATT
TAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8535.79 Da
• Isoelectric Point: 11.0482

>AT224492 WP_009503866.1 NZ_CP086817:c3880-3653 [Pseudosulfitobacter pseudonitzschiae]
MQVAKWGNSLAVRLPAELVRELGLKEGDQIDLVKDDGRVRVRRLARADEVLTGLRRFRGKLSAAERLSRDDAHER

Nucleotide

• Length: 228 bp

>AT224492 NZ_CP120336:28612-28896 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGCAAACTACAATCCGCAAATCTGTGCGTAATAAACAAATAAATATACGGGCTACTGATGAGGAACGTGCTGTGATTGA
TTATGCAGCGAGCCTGGTCAGTAAAAACCGTACCGACTTCATCATAGAAAAGGCTGTGAGCGAGGCCCAGAATATCATTC
TGGATCAACGCGTTTTTGTTCTGGATGATGCCCGCTATCAGGCTTTTATTAAACAACTTGAAGCTCCTGTACAGAATACT
GAAGGTCGTCAGCGCCTGATGGATGTGAAACCTGAATGGAAATAA