Detailed information of TA system TA224490

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 718688..719153
• Replicon: chromosome
• Accession: NZ_CP086816
• Organism: Pseudosulfitobacter pseudonitzschiae strain TC12-52

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LOE26_RS03465
• Protein ID: WP_224557762.1
• Coordinates: 718688..718951 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LOE26_RS03470
• Protein ID: WP_224557764.1
• Coordinates: 718938..719153 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE26_RS03440 (LOE26_03440)	714404..715954	-	1551	WP_224557015.1	restriction endonuclease subunit S	-
LOE26_RS03445 (LOE26_03445)	715951..716247	-	297	WP_224557153.1	type I restriction-modification enzyme R subunit C-terminal domain-containing protein	-
LOE26_RS03450 (LOE26_03450)	716141..716629	-	489	WP_229938596.1	DEAD/DEAH box helicase family protein	-
LOE26_RS03455 (LOE26_03455)	716855..717964	-	1110	WP_224558030.1	IS110 family transposase	-
LOE26_RS03460 (LOE26_03460)	718057..718500	-	444	WP_224557760.1	hypothetical protein	-
LOE26_RS03465 (LOE26_03465)	718688..718951	+	264	WP_224557762.1	BrnT family toxin	Toxin
LOE26_RS03470 (LOE26_03470)	718938..719153	+	216	WP_224557764.1	CopG family antitoxin	Antitoxin
LOE26_RS03475 (LOE26_03475)	719506..719730	-	225	WP_224557765.1	hypothetical protein	-
LOE26_RS03480 (LOE26_03480)	719898..720278	-	381	WP_224557766.1	helix-turn-helix domain-containing protein	-
LOE26_RS03485 (LOE26_03485)	720459..721430	-	972	WP_224557767.1	hypothetical protein	-
LOE26_RS03490 (LOE26_03490)	721423..722607	-	1185	WP_224557768.1	site-specific integrase	-
LOE26_RS03500 (LOE26_03500)	722985..723683	-	699	WP_037931342.1	SURF1 family protein	-
LOE26_RS03505 (LOE26_03505)	723680..724045	-	366	WP_028957811.1	cytochrome o ubiquinol oxidase subunit IV	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	710307..722607	12300

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-80)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10254.52 Da
• Isoelectric Point: 5.6550

>T224490 WP_224557762.1 NZ_CP086816:718688-718951 [Pseudosulfitobacter pseudonitzschiae]
MKFEYDPDKSAGNKAKHGIDFDEAQALWEDPWLLEAPARTDDEPRFFAIGMIKERHWSAVCTYRGDIIRIISVRRARKQE
IEHYEST

Nucleotide

• Length: 264 bp

>T224490 NZ_CP120335:4667584-4667895 [Salmonella enterica subsp. enterica serovar Muenchen]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATACTATCGGGGTAATAAAGAATGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8265.40 Da
• Isoelectric Point: 10.6538

>AT224490 WP_224557764.1 NZ_CP086816:718938-719153 [Pseudosulfitobacter pseudonitzschiae]
MKAREFDQKFDTGENVSDLVDWSKARRTNTQTKRVNVDFPSWVVSGLDQKAKQLGVTRQSLIKLWIAERLQ

Nucleotide

• Length: 216 bp

>AT224490 NZ_CP120335:4667892-4668338 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAACCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAATCACTCAAC
CAACACCTGGAGAAAACACCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTTAAACTACCAGCGGATGCCTTCATCGAGTAG