Detailed information of TA system TA022449

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1609260..1609898
• Replicon: chromosome
• Accession: NC_009801
• Organism: Escherichia coli E24377A

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: ECE24377A_RS09660
• Protein ID: WP_000813794.1
• Coordinates: 1609260..1609436 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: ECE24377A_RS09665
• Protein ID: WP_001270286.1
• Coordinates: 1609482..1609898 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECE24377A_RS09640	1604879..1606093	-	1215	WP_001307190.1	BenE family transporter YdcO	-
ECE24377A_RS09645	1606146..1606682	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
ECE24377A_RS09650	1606755..1608716	+	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
ECE24377A_RS09655	1608808..1609038	-	231	WP_000494244.1	YncJ family protein	-
ECE24377A_RS09660	1609260..1609436	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
ECE24377A_RS09665	1609482..1609898	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
ECE24377A_RS09670	1609977..1611383	+	1407	WP_000760629.1	PLP-dependent aminotransferase family protein	-
ECE24377A_RS09675	1611628..1612773	+	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
ECE24377A_RS09680	1612791..1613804	+	1014	WP_000220411.1	ABC transporter ATP-binding protein	-
ECE24377A_RS09685	1613805..1614746	+	942	WP_001251315.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	vgrG/tssI / rhs/PAAR / espR1 / sodB	1530882..1862356	331474

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T22449 WP_000813794.1 NC_009801:1609260-1609436 [Escherichia coli O139:H28 str. E24377A]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T22449 NC_009801:1609260-1609436 [Escherichia coli O139:H28 str. E24377A]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT22449 WP_001270286.1 NC_009801:1609482-1609898 [Escherichia coli O139:H28 str. E24377A]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT22449 NC_009801:1609482-1609898 [Escherichia coli O139:H28 str. E24377A]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGTAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB