Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/PemK-MazE |
| Location | 240669..241210 | Replicon | plasmid P3 |
| Accession | NZ_CP086811 | ||
| Organism | Pseudosulfitobacter pseudonitzschiae strain TC12-54 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | A0A073IW87 |
| Locus tag | LOE27_RS21935 | Protein ID | WP_037931244.1 |
| Coordinates | 240884..241210 (+) | Length | 109 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | A0A073IWP4 |
| Locus tag | LOE27_RS21930 | Protein ID | WP_037931247.1 |
| Coordinates | 240669..240887 (+) | Length | 73 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LOE27_RS21910 (LOE27_21890) | 235926..236933 | + | 1008 | WP_081855484.1 | PaaX family transcriptional regulator C-terminal domain-containing protein | - |
| LOE27_RS21915 (LOE27_21895) | 237168..238715 | + | 1548 | WP_073194592.1 | AMP-binding protein | - |
| LOE27_RS21920 (LOE27_21900) | 238770..239234 | + | 465 | WP_051694690.1 | MaoC/PaaZ C-terminal domain-containing protein | - |
| LOE27_RS21925 (LOE27_21905) | 239238..240371 | + | 1134 | WP_037931249.1 | alpha/beta fold hydrolase | - |
| LOE27_RS21930 (LOE27_21910) | 240669..240887 | + | 219 | WP_037931247.1 | antitoxin MazE family protein | Antitoxin |
| LOE27_RS21935 (LOE27_21915) | 240884..241210 | + | 327 | WP_037931244.1 | type II toxin-antitoxin system PemK/MazF family toxin | Toxin |
| LOE27_RS21940 (LOE27_21920) | 241384..241740 | - | 357 | Protein_232 | ATP-binding protein | - |
| LOE27_RS21945 (LOE27_21925) | 241730..243302 | - | 1573 | Protein_233 | IS21 family transposase | - |
| LOE27_RS21950 (LOE27_21930) | 243638..243961 | - | 324 | WP_027238707.1 | helix-turn-helix domain-containing protein | - |
| LOE27_RS21955 (LOE27_21935) | 244124..244531 | + | 408 | WP_157802081.1 | DoxX family protein | - |
| LOE27_RS21960 (LOE27_21940) | 244595..245410 | + | 816 | WP_224556226.1 | class III extradiol ring-cleavage dioxygenase | - |
| LOE27_RS21965 (LOE27_21945) | 245419..245916 | + | 498 | WP_037931962.1 | DUF5519 family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..290595 | 290595 | |
| - | flank | IS/Tn | - | - | 241730..243016 | 1286 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 109 a.a. Molecular weight: 11610.50 Da Isoelectric Point: 8.8545
>T224487 WP_037931244.1 NZ_CP086811:240884-241210 [Pseudosulfitobacter pseudonitzschiae]
VRRGELVTVALQGDHGKPRPALVIQSDRFPDTATTTVLLVTSTLVDAPLIRLTVEPSAENGLRMRSQIMIDKAVTVRTDK
LGKAFGQLDDTAMIEVNRALALFLGVAG
VRRGELVTVALQGDHGKPRPALVIQSDRFPDTATTTVLLVTSTLVDAPLIRLTVEPSAENGLRMRSQIMIDKAVTVRTDK
LGKAFGQLDDTAMIEVNRALALFLGVAG
Download Length: 327 bp
>T224487 NZ_CP120335:c4165817-4165479 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGAAAATCCTTTCTGCAAATCAGCGGGTGGCGAAGCCATGCCCGCCGCCTGTACTTGTCTGGCAAACGCTACTGACACG
CCTGCTGGAGCAACACTATGGCCTGTCGCTGAACGATACGCCGTTCAGTGATGAAACGGTTATTCAGGAGCATATCAACG
CCGGTATCACGCTGGCGGATGCAGTTAACTTTCTGGTGGAAAAGTACGAACTGGTTCGTATCGATCGCAGAGGGTTTAAC
TGTCAGGAACAGTCGCCTTACCTTCGGGCGGTTGATATTTTACGGGCGCGACAGGCTACTGGTCTGTTGCGGCAAAACCA
GCTTCCTTCGACACGCTGA
ATGAAAATCCTTTCTGCAAATCAGCGGGTGGCGAAGCCATGCCCGCCGCCTGTACTTGTCTGGCAAACGCTACTGACACG
CCTGCTGGAGCAACACTATGGCCTGTCGCTGAACGATACGCCGTTCAGTGATGAAACGGTTATTCAGGAGCATATCAACG
CCGGTATCACGCTGGCGGATGCAGTTAACTTTCTGGTGGAAAAGTACGAACTGGTTCGTATCGATCGCAGAGGGTTTAAC
TGTCAGGAACAGTCGCCTTACCTTCGGGCGGTTGATATTTTACGGGCGCGACAGGCTACTGGTCTGTTGCGGCAAAACCA
GCTTCCTTCGACACGCTGA
Antitoxin
Download Length: 73 a.a. Molecular weight: 8175.17 Da Isoelectric Point: 5.8017
>AT224487 WP_037931247.1 NZ_CP086811:240669-240887 [Pseudosulfitobacter pseudonitzschiae]
MPTSVSHRVQKRRDALRAAGLRPVQIWVPDTRRPGFEAECRRQAAIVAKADRADDTLNDFLDASLVDVDDWE
MPTSVSHRVQKRRDALRAAGLRPVQIWVPDTRRPGFEAECRRQAAIVAKADRADDTLNDFLDASLVDVDDWE
Download Length: 219 bp
>AT224487 NZ_CP120335:c4166171-4165842 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGGATAAAGAAACCGACACAGTACAGTGGGGACTCAACCGACCTGGTTCTCCTCAATTTAGCGCACGCCTGGTACAAGA
AGGCAAACGCCTACATTATTTAGCTGACCGCGCCGGGATAACAGGCGCTTTCAATGAAGAAGAGTGTCACCAACTGAATG
CCGCATTTCCGCACTTCGTAAAACAAATGGAGCTGATGCTGCTCTCTGGTGAGCTTAACCCCCGCCATGCATATCGCGTA
ACGCTGTACCACAACGAACTGACCTGTGAAGCAGATACGCTCGGTAGCTGTGGCTACGTTTATATAGCGATTTATCCCAC
TCAACGCTAA
ATGGATAAAGAAACCGACACAGTACAGTGGGGACTCAACCGACCTGGTTCTCCTCAATTTAGCGCACGCCTGGTACAAGA
AGGCAAACGCCTACATTATTTAGCTGACCGCGCCGGGATAACAGGCGCTTTCAATGAAGAAGAGTGTCACCAACTGAATG
CCGCATTTCCGCACTTCGTAAAACAAATGGAGCTGATGCTGCTCTCTGGTGAGCTTAACCCCCGCCATGCATATCGCGTA
ACGCTGTACCACAACGAACTGACCTGTGAAGCAGATACGCTCGGTAGCTGTGGCTACGTTTATATAGCGATTTATCCCAC
TCAACGCTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A073IW87 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A073IWP4 |