Detailed information of TA system TA224483

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 89332..89797
• Replicon: chromosome
• Accession: NZ_CP086808
• Organism: Pseudosulfitobacter pseudonitzschiae strain TC12-54

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LOE27_RS00465
• Protein ID: WP_224557762.1
• Coordinates: 89534..89797 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LOE27_RS00460
• Protein ID: WP_224557764.1
• Coordinates: 89332..89547 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE27_RS00425 (LOE27_00420)	84440..84805	+	366	WP_028957811.1	cytochrome o ubiquinol oxidase subunit IV	-
LOE27_RS00430 (LOE27_00425)	84802..85500	+	699	WP_037931342.1	SURF1 family protein	-
LOE27_RS00440 (LOE27_00435)	85878..87062	+	1185	WP_224557768.1	site-specific integrase	-
LOE27_RS00445 (LOE27_00440)	87055..88026	+	972	WP_224557767.1	hypothetical protein	-
LOE27_RS00450 (LOE27_00445)	88207..88587	+	381	WP_224557766.1	helix-turn-helix domain-containing protein	-
LOE27_RS00455 (LOE27_00450)	88755..88979	+	225	WP_224557765.1	hypothetical protein	-
LOE27_RS00460 (LOE27_00455)	89332..89547	-	216	WP_224557764.1	CopG family antitoxin	Antitoxin
LOE27_RS00465 (LOE27_00460)	89534..89797	-	264	WP_224557762.1	BrnT family toxin	Toxin
LOE27_RS00470 (LOE27_00465)	89985..90428	+	444	WP_224557760.1	hypothetical protein	-
LOE27_RS00475 (LOE27_00470)	90521..91630	+	1110	WP_224558030.1	IS110 family transposase	-
LOE27_RS00480 (LOE27_00475)	91856..92344	+	489	WP_229938596.1	DEAD/DEAH box helicase family protein	-
LOE27_RS00485 (LOE27_00480)	92268..92534	+	267	WP_229938971.1	type I restriction-modification enzyme R subunit C-terminal domain-containing protein	-
LOE27_RS00490 (LOE27_00485)	92531..94081	+	1551	WP_224557015.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	87232..98391	11159

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-80)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10254.52 Da
• Isoelectric Point: 5.6550

>T224483 WP_224557762.1 NZ_CP086808:c89797-89534 [Pseudosulfitobacter pseudonitzschiae]
MKFEYDPDKSAGNKAKHGIDFDEAQALWEDPWLLEAPARTDDEPRFFAIGMIKERHWSAVCTYRGDIIRIISVRRARKQE
IEHYEST

Nucleotide

• Length: 264 bp

>T224483 NZ_CP120335:c1017838-1017311 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATACGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGCGGCGTAGCCCTGCTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8265.40 Da
• Isoelectric Point: 10.6538

>AT224483 WP_224557764.1 NZ_CP086808:c89547-89332 [Pseudosulfitobacter pseudonitzschiae]
MKAREFDQKFDTGENVSDLVDWSKARRTNTQTKRVNVDFPSWVVSGLDQKAKQLGVTRQSLIKLWIAERLQ

Nucleotide

• Length: 216 bp

>AT224483 NZ_CP120335:c1018125-1017835 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGGCTGTCGTTACGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGTGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCTGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA