Detailed information of TA system TA224481

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 13741..14353
• Replicon: plasmid P5
• Accession: NZ_CP086803
• Organism: Pseudosulfitobacter pseudonitzschiae strain TT37-47

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LOE37_RS21605
• Protein ID: WP_028288627.1
• Coordinates: 13741..14136 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A921TG38
• Locus tag: LOE37_RS21610
• Protein ID: WP_009503866.1
• Coordinates: 14126..14353 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE37_RS21585 (LOE37_21580)	10260..10871	-	612	WP_197919471.1	SMC-Scp complex subunit ScpB	-
LOE37_RS21590 (LOE37_21585)	10919..11803	-	885	WP_037239259.1	DUF1403 family protein	-
LOE37_RS21595 (LOE37_21590)	11930..13069	+	1140	WP_229940649.1	tyrosine-type recombinase/integrase	-
LOE37_RS21600 (LOE37_21595)	13171..13662	+	492	Protein_12	recombinase family protein	-
LOE37_RS21605 (LOE37_21600)	13741..14136	-	396	WP_028288627.1	PIN domain-containing protein	Toxin
LOE37_RS21610 (LOE37_21605)	14126..14353	-	228	WP_009503866.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LOE37_RS21615 (LOE37_21610)	14562..15755	+	1194	WP_009503867.1	plasmid partitioning protein RepA	-
LOE37_RS21620 (LOE37_21615)	15739..16671	+	933	WP_028288626.1	plasmid partitioning protein RepB	-
LOE37_RS21625 (LOE37_21620)	16885..18096	+	1212	WP_050525470.1	plasmid replication protein RepC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..110690	110690

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-113)

Antitoxin

• Antitoxin PemI-like (2-47)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14212.23 Da
• Isoelectric Point: 4.1653

>T224481 WP_028288627.1 NZ_CP086803:c14136-13741 [Pseudosulfitobacter pseudonitzschiae]
MSVEFADTNVVLYLLDDGPKADRAEVILGQGPRISVQVLNESLVNCRRKAGLGWEEAAAFLEGVRALCPVEDLTVQTHDV
GRALAERYGFSIYDAMIVASALVAGCTTLWSEDMQDGLLVEGQLRIVNPFA

Nucleotide

• Length: 396 bp

>T224481 NZ_CP120335:311879-312247 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTATCGGGTACTCAATCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCACTATATCCCGTGGGTATATCTTCAATGATGGTAACAAACGCACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAACAAATTGCCGTTCGCTTACGTGCCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8535.79 Da
• Isoelectric Point: 11.0482

>AT224481 WP_009503866.1 NZ_CP086803:c14353-14126 [Pseudosulfitobacter pseudonitzschiae]
MQVAKWGNSLAVRLPAELVRELGLKEGDQIDLVKDDGRVRVRRLARADEVLTGLRRFRGKLSAAERLSRDDAHER

Nucleotide

• Length: 228 bp

>AT224481 NZ_CP120335:311661-311882 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGCGCGGTAACGGCGGGCCCTGTTAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGAAGCTGGCGGATAAATGA