Detailed information of TA system TA224473

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 43609..44150
• Replicon: plasmid P6
• Accession: NZ_CP086796
• Organism: Pseudosulfitobacter pseudonitzschiae strain TT37-67

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A073IW87
• Locus tag: LOE38_RS22975
• Protein ID: WP_037931244.1
• Coordinates: 43824..44150 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A073IWP4
• Locus tag: LOE38_RS22970
• Protein ID: WP_037931247.1
• Coordinates: 43609..43827 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE38_RS22950 (LOE38_22935)	38866..39873	+	1008	WP_081855484.1	PaaX family transcriptional regulator C-terminal domain-containing protein	-
LOE38_RS22955 (LOE38_22940)	40108..41655	+	1548	WP_073194592.1	AMP-binding protein	-
LOE38_RS22960 (LOE38_22945)	41710..42174	+	465	WP_051694690.1	MaoC/PaaZ C-terminal domain-containing protein	-
LOE38_RS22965 (LOE38_22950)	42178..43311	+	1134	WP_037931249.1	alpha/beta fold hydrolase	-
LOE38_RS22970 (LOE38_22955)	43609..43827	+	219	WP_037931247.1	antitoxin MazE family protein	Antitoxin
LOE38_RS22975 (LOE38_22960)	43824..44150	+	327	WP_037931244.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LOE38_RS22980 (LOE38_22965)	44324..44680	-	357	Protein_47	ATP-binding protein	-
LOE38_RS22985 (LOE38_22970)	44670..46242	-	1573	Protein_48	IS21 family transposase	-
LOE38_RS22990 (LOE38_22975)	46578..46901	-	324	WP_027238707.1	helix-turn-helix domain-containing protein	-
LOE38_RS22995 (LOE38_22980)	47064..47471	+	408	WP_157802081.1	DoxX family protein	-
LOE38_RS23000 (LOE38_22985)	47535..48350	+	816	WP_224556226.1	class III extradiol ring-cleavage dioxygenase	-
LOE38_RS23005 (LOE38_22990)	48359..48856	+	498	WP_037931962.1	DUF5519 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..290596	290596
	-	flank	IS/Tn	-	-	44670..45956	1286

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-105)

Antitoxin

• Antitoxin MazE-like (7-71)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11610.50 Da
• Isoelectric Point: 8.8545

>T224473 WP_037931244.1 NZ_CP086796:43824-44150 [Pseudosulfitobacter pseudonitzschiae]
VRRGELVTVALQGDHGKPRPALVIQSDRFPDTATTTVLLVTSTLVDAPLIRLTVEPSAENGLRMRSQIMIDKAVTVRTDK
LGKAFGQLDDTAMIEVNRALALFLGVAG

Nucleotide

• Length: 327 bp

>T224473 NZ_CP120333:c4165976-4165638 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGAAAATCCTTTCTGCAAATCAGCGGGTGGCGAAGCCATGCCCGCCGCCTGTACTTGTCTGGCAAACGCTACTGACACG
CCTGCTGGAGCAACACTATGGCCTGTCGCTGAACGATACGCCGTTCAGTGATGAAACGGTTATTCAGGAGCATATCAACG
CCGGTATCACGCTGGCGGATGCAGTTAACTTTCTGGTGGAAAAGTACGAACTGGTTCGTATCGATCGCAGAGGGTTTAAC
TGTCAGGAACAGTCGCCTTACCTTCGGGCGGTTGATATTTTACGGGCGCGACAGGCTACTGGTCTGTTGCGGCAAAACCA
GCTTCCTTCGACACGCTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8175.17 Da
• Isoelectric Point: 5.8017

>AT224473 WP_037931247.1 NZ_CP086796:43609-43827 [Pseudosulfitobacter pseudonitzschiae]
MPTSVSHRVQKRRDALRAAGLRPVQIWVPDTRRPGFEAECRRQAAIVAKADRADDTLNDFLDASLVDVDDWE

Nucleotide

• Length: 219 bp

>AT224473 NZ_CP120333:c4166330-4166001 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGGATAAAGAAACCGACACAGTACAGTGGGGACTCAACCGACCTGGTTCTCCTCAATTTAGCGCACGCCTGGTACAAGA
AGGCAAACGCCTACATTATTTAGCTGACCGCGCCGGGATAACAGGCGCTTTCAATGAAGAAGAGTGTCACCAACTGAATG
CCGCATTTCCGCACTTCGTAAAACAAATGGAGCTGATGCTGCTCTCTGGTGAGCTTAACCCCCGCCATGCATATCGCGTA
ACGCTGTACCACAACGAACTGACCTGTGAAGCAGATACGCTCGGTAGCTGTGGCTACGTTTATATAGCGATTTATCCCAC
TCAACGCTAA

Structures

Toxin

• AlphaFold DB: A0A073IW87

Antitoxin

• AlphaFold DB: A0A073IWP4