Detailed information of TA system TA224466

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 135758..136432
• Replicon: plasmid P5
• Accession: NZ_CP086788
• Organism: Pseudosulfitobacter pseudonitzschiae strain TT37-68

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LOE39_RS22735
• Protein ID: WP_224557924.1
• Coordinates: 136010..136432 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LOE39_RS22730
• Protein ID: WP_229939240.1
• Coordinates: 135758..136006 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE39_RS22710 (LOE39_22700)	131671..132315	+	645	WP_224557929.1	type IV secretion system protein	-
LOE39_RS22715 (LOE39_22705)	132319..133050	+	732	WP_224557928.1	TrbG/VirB9 family P-type conjugative transfer protein	-
LOE39_RS22720 (LOE39_22710)	133053..134510	+	1458	WP_224557927.1	TrbI/VirB10 family protein	-
LOE39_RS22725 (LOE39_22715)	134621..135610	+	990	WP_224557926.1	P-type DNA transfer ATPase VirB11	-
LOE39_RS22730 (LOE39_22720)	135758..136006	+	249	WP_229939240.1	plasmid stabilization protein	Antitoxin
LOE39_RS22735 (LOE39_22725)	136010..136432	+	423	WP_224557924.1	type II toxin-antitoxin system VapC family toxin	Toxin
LOE39_RS22740 (LOE39_22730)	136429..139179	+	2751	WP_224557923.1	lactate dehydrogenase	-
LOE39_RS22745 (LOE39_22735)	139176..140234	+	1059	WP_224557922.1	HEPN domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..157664	157664

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15255.26 Da
• Isoelectric Point: 4.3767

>T224466 WP_224557924.1 NZ_CP086788:136010-136432 [Pseudosulfitobacter pseudonitzschiae]
MIILDTNVICELLRPTPEPRIEQWLSAQDGLNVYLTSISEAELRYGLANMGNGKRRAALIDAVDRILREDLAGRILPFDS
DAAQAYASIAATRRDAGRPIAQADCQIASIAHACGATVATRNTPDFEGCEIDLINPWTTE

Nucleotide

• Length: 423 bp

>T224466 NZ_CP120332:50117-50422 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTACCGCCTGTTTGTGGATGTGCAGAGCGACATCGTCGATAC
CCCTGGCCGGCGAATGGTGATCCCGCTGGCCAGTGCCCGTCTGCTGTCGGATAAGGTCTCCCGCGAACTTTATCCGGTGG
TGCATATCGGGGATGAGAGCTGGCGTCTGATGACCACGGATATGGCCAGTGTCCCGGCCTCATTCATCGGGGAAGAGGTG
GCCGAGCTCAGCCACAGGGAGAACGACATCAAAAACGCCATTAACCTGATGTTCTGGGGGATCTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9206.46 Da
• Isoelectric Point: 6.7281

>AT224466 WP_229939240.1 NZ_CP086788:135758-136006 [Pseudosulfitobacter pseudonitzschiae]
MASITIRNLDDDVKRRLRIRAAEHGRSMEEEAREILRQVVGDAKPNHDLAAAIRARVGPLGGVELDLPQREAMHEPPTFD
RA

Nucleotide

• Length: 249 bp

>AT224466 NZ_CP120332:49897-50115 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGAAGCAGCGTATTACTGTCACCATAGACAGTGACAGCTATCAGTTACTCAAGTCCGCAGACGTGAATATCTCCGGCCT
GGTAAATACTGCCATGCAGAAGGAGGCCCGCCGTCTGCGTGCGGAACGCTGGCAGGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAGCGAACGGCTCCTTTGCAGACGAAAACAGGGACTGGTGA