Detailed information of TA system TA224465

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 5351..5963
• Replicon: plasmid P2
• Accession: NZ_CP086785
• Organism: Pseudosulfitobacter pseudonitzschiae strain TT37-68

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LOE39_RS20100
• Protein ID: WP_028288627.1
• Coordinates: 5568..5963 (+)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A921TG38
• Locus tag: LOE39_RS20095
• Protein ID: WP_009503866.1
• Coordinates: 5351..5578 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE39_RS20080 (LOE39_20070)	1608..2819	-	1212	WP_050525470.1	plasmid replication protein RepC	-
LOE39_RS20085 (LOE39_20075)	3033..3965	-	933	WP_028288626.1	plasmid partitioning protein RepB	-
LOE39_RS20090 (LOE39_20080)	3949..5142	-	1194	WP_009503867.1	plasmid partitioning protein RepA	-
LOE39_RS20095 (LOE39_20085)	5351..5578	+	228	WP_009503866.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LOE39_RS20100 (LOE39_20090)	5568..5963	+	396	WP_028288627.1	PIN domain-containing protein	Toxin
LOE39_RS20105 (LOE39_20095)	6042..6533	-	492	Protein_6	recombinase family protein	-
LOE39_RS20110 (LOE39_20100)	6635..7774	-	1140	WP_229940649.1	tyrosine-type recombinase/integrase	-
LOE39_RS20115 (LOE39_20105)	7901..8785	+	885	WP_037239259.1	DUF1403 family protein	-
LOE39_RS20120 (LOE39_20110)	8833..9444	+	612	WP_197919471.1	SMC-Scp complex subunit ScpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..110568	110568

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-113)

Antitoxin

• Antitoxin PemI-like (2-47)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14212.23 Da
• Isoelectric Point: 4.1653

>T224465 WP_028288627.1 NZ_CP086785:5568-5963 [Pseudosulfitobacter pseudonitzschiae]
MSVEFADTNVVLYLLDDGPKADRAEVILGQGPRISVQVLNESLVNCRRKAGLGWEEAAAFLEGVRALCPVEDLTVQTHDV
GRALAERYGFSIYDAMIVASALVAGCTTLWSEDMQDGLLVEGQLRIVNPFA

Nucleotide

• Length: 396 bp

>T224465 NZ_CP120332:c49046-48630 [Salmonella enterica subsp. enterica serovar Muenchen]
GTGAAGAAAATGTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCCGAAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCACCGTATTGTGGTCTCGGCCATCACCTACTCAGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGTCACATTGAACTGGTTGACGCGTTCTGCGCCCGCCTCGATGCTATCCTGCCCTGGGACCGGGCC
GCGGTGAACGCCACCACGGAGATTAAGGTGGCGCTACGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCGATCGC
CGGGCACGCTATTGCCGCCGGCGCGGTACTGGTGACAAATAATACGCGGGAGTTTGAGCGGGTGCCGGGTCTTGTTCTGG
AAGACTGGGGAAGATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8535.79 Da
• Isoelectric Point: 11.0482

>AT224465 WP_009503866.1 NZ_CP086785:5351-5578 [Pseudosulfitobacter pseudonitzschiae]
MQVAKWGNSLAVRLPAELVRELGLKEGDQIDLVKDDGRVRVRRLARADEVLTGLRRFRGKLSAAERLSRDDAHER

Nucleotide

• Length: 228 bp

>AT224465 NZ_CP120332:c49273-49043 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGAGAACCGTATCTATTTTTAAAAACGGCAACAACCGCGCCATCCGCCTTCCCCGCGATCTGGATTTTGATGGAGTGAG
CGAGCTGGAAATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCTGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTCG
AAAAAGCTGACCCGGGCTTTATGGCGGAGCGTGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA