Detailed information of TA system TA224458

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 3193832..3194297
• Replicon: chromosome
• Accession: NZ_CP086775
• Organism: Pseudosulfitobacter pseudonitzschiae strain TT37-69

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LOE40_RS16060
• Protein ID: WP_224557762.1
• Coordinates: 3194034..3194297 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LOE40_RS16055
• Protein ID: WP_224557764.1
• Coordinates: 3193832..3194047 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE40_RS16020 (LOE40_16005)	3188940..3189305	+	366	WP_028957811.1	cytochrome o ubiquinol oxidase subunit IV	-
LOE40_RS16025 (LOE40_16010)	3189302..3190000	+	699	WP_037931342.1	SURF1 family protein	-
LOE40_RS16035 (LOE40_16020)	3190378..3191562	+	1185	WP_224557768.1	site-specific integrase	-
LOE40_RS16040 (LOE40_16025)	3191555..3192526	+	972	WP_224557767.1	hypothetical protein	-
LOE40_RS16045 (LOE40_16030)	3192707..3193087	+	381	WP_224557766.1	helix-turn-helix domain-containing protein	-
LOE40_RS16050 (LOE40_16035)	3193255..3193479	+	225	WP_224557765.1	hypothetical protein	-
LOE40_RS16055 (LOE40_16040)	3193832..3194047	-	216	WP_224557764.1	CopG family antitoxin	Antitoxin
LOE40_RS16060 (LOE40_16045)	3194034..3194297	-	264	WP_224557762.1	BrnT family toxin	Toxin
LOE40_RS16065 (LOE40_16050)	3194485..3194928	+	444	WP_224557760.1	hypothetical protein	-
LOE40_RS16070 (LOE40_16055)	3195021..3196130	+	1110	WP_224558030.1	IS110 family transposase	-
LOE40_RS16075 (LOE40_16060)	3196356..3196844	+	489	WP_229938596.1	DEAD/DEAH box helicase family protein	-
LOE40_RS16080 (LOE40_16065)	3196768..3197034	+	267	WP_229938971.1	type I restriction-modification enzyme R subunit C-terminal domain-containing protein	-
LOE40_RS16085 (LOE40_16070)	3197031..3198581	+	1551	WP_224557015.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3191732..3202891	11159

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-80)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10254.52 Da
• Isoelectric Point: 5.6550

>T224458 WP_224557762.1 NZ_CP086775:c3194297-3194034 [Pseudosulfitobacter pseudonitzschiae]
MKFEYDPDKSAGNKAKHGIDFDEAQALWEDPWLLEAPARTDDEPRFFAIGMIKERHWSAVCTYRGDIIRIISVRRARKQE
IEHYEST

Nucleotide

• Length: 264 bp

>T224458 NZ_CP120331:3723643-3723918 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGAATGCCTCCGGCGTTAGCGTCCGCCATATCAACAGCGAAACCTGCATGACCGCCTGTTACTCACAAATCCCCAGCCA
GCACCTTAAGGGTGACTGGCAGGAAGAAGCGGGATTTGAGACCGGGCACGGCGTCACCGTGAAGATCTCCGAGGGGTGTT
TGATCCTGATTGCAGAGACGGACGAAGTGAGGGATCTACGGAAGGAGCTTTATCAGGTGAAAAAGTCGATGAAGCATATT
AAGGCGGGGGTAAATAATGTGGTGAACGGGGATTAA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8265.40 Da
• Isoelectric Point: 10.6538

>AT224458 WP_224557764.1 NZ_CP086775:c3194047-3193832 [Pseudosulfitobacter pseudonitzschiae]
MKAREFDQKFDTGENVSDLVDWSKARRTNTQTKRVNVDFPSWVVSGLDQKAKQLGVTRQSLIKLWIAERLQ

Nucleotide

• Length: 216 bp

>AT224458 NZ_CP120331:c3723908-3723486 [Salmonella enterica subsp. enterica serovar Muenchen]
TTCACCACATTATTTACCCCCGCCTTAATATGCTTCATCGACTTTTTCACCTGATAAAGCTCCTTCCGTAGATCCCTCAC
TTCGTCCGTCTCTGCAATCAGGATCAAACACCCCTCGGAGATCTTCACGGTGACGCCGTGCCCGGTCTCAAATCCCGCTT
CTTCCTGCCAGTCACCCTTAAGGTGCTGGCTGGGGATTTGTGAGTAACAGGCGGTCATGCAGGTTTCGCTGTTGATATGG
CGGACGCTAACGCCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTATTTATCCGCCGGATGCTGGCTGATTGTGGA
GCTCAGGGTGAGTGAGTATGGGCGCGACATCCTGGCACCGCGCCTCCCTCTCCCCGGCCAGCCCCGGCCGGTGATGAGCA
GCCGGCTGCCGGGGCCGTATTTT