Detailed information of TA system TA224454

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 23504..24045
• Replicon: plasmid P6
• Accession: NZ_CP086770
• Organism: Pseudosulfitobacter pseudonitzschiae strain TT37-74

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A073IW87
• Locus tag: LOE41_RS22075
• Protein ID: WP_037931244.1
• Coordinates: 23504..23830 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A073IWP4
• Locus tag: LOE41_RS22080
• Protein ID: WP_037931247.1
• Coordinates: 23827..24045 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE41_RS22045 (LOE41_22040)	18798..19295	-	498	WP_037931962.1	DUF5519 family protein	-
LOE41_RS22050 (LOE41_22045)	19304..20119	-	816	WP_224556226.1	class III extradiol ring-cleavage dioxygenase	-
LOE41_RS22055 (LOE41_22050)	20183..20590	-	408	WP_157802081.1	DoxX family protein	-
LOE41_RS22060 (LOE41_22055)	20753..21076	+	324	WP_027238707.1	helix-turn-helix domain-containing protein	-
LOE41_RS22065 (LOE41_22060)	21412..22984	+	1573	Protein_25	IS21 family transposase	-
LOE41_RS22070 (LOE41_22065)	22974..23330	+	357	Protein_26	ATP-binding protein	-
LOE41_RS22075 (LOE41_22070)	23504..23830	-	327	WP_037931244.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LOE41_RS22080 (LOE41_22075)	23827..24045	-	219	WP_037931247.1	antitoxin MazE family protein	Antitoxin
LOE41_RS22085 (LOE41_22080)	24343..25476	-	1134	WP_037931249.1	alpha/beta fold hydrolase	-
LOE41_RS22090 (LOE41_22085)	25480..25944	-	465	WP_051694690.1	MaoC/PaaZ C-terminal domain-containing protein	-
LOE41_RS22095 (LOE41_22090)	25999..27546	-	1548	WP_073194592.1	AMP-binding protein	-
LOE41_RS22100 (LOE41_22095)	27781..28788	-	1008	WP_081855484.1	PaaX family transcriptional regulator C-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..194797	194797
	-	flank	IS/Tn	-	-	21698..22984	1286

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-105)

Antitoxin

• Antitoxin MazE-like (7-71)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11610.50 Da
• Isoelectric Point: 8.8545

>T224454 WP_037931244.1 NZ_CP086770:c23830-23504 [Pseudosulfitobacter pseudonitzschiae]
VRRGELVTVALQGDHGKPRPALVIQSDRFPDTATTTVLLVTSTLVDAPLIRLTVEPSAENGLRMRSQIMIDKAVTVRTDK
LGKAFGQLDDTAMIEVNRALALFLGVAG

Nucleotide

• Length: 327 bp

>T224454 NZ_CP120331:854957-855370 [Salmonella enterica subsp. enterica serovar Muenchen]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTAATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGTTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8175.17 Da
• Isoelectric Point: 5.8017

>AT224454 WP_037931247.1 NZ_CP086770:c24045-23827 [Pseudosulfitobacter pseudonitzschiae]
MPTSVSHRVQKRRDALRAAGLRPVQIWVPDTRRPGFEAECRRQAAIVAKADRADDTLNDFLDASLVDVDDWE

Nucleotide

• Length: 219 bp

>AT224454 NZ_CP120331:854710-854976 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A073IW87

Antitoxin

• AlphaFold DB: A0A073IWP4