Detailed information of TA system TA224450

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 1520901..1521366
• Replicon: chromosome
• Accession: NZ_CP086764
• Organism: Pseudosulfitobacter pseudonitzschiae strain TT37-74

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LOE41_RS07780
• Protein ID: WP_224557762.1
• Coordinates: 1520901..1521164 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LOE41_RS07785
• Protein ID: WP_224557764.1
• Coordinates: 1521151..1521366 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE41_RS07755 (LOE41_07740)	1516617..1518167	-	1551	WP_224557015.1	restriction endonuclease subunit S	-
LOE41_RS07760 (LOE41_07745)	1518164..1518460	-	297	WP_224557153.1	type I restriction-modification enzyme R subunit C-terminal domain-containing protein	-
LOE41_RS07765 (LOE41_07750)	1518354..1518842	-	489	WP_229938596.1	DEAD/DEAH box helicase family protein	-
LOE41_RS07770 (LOE41_07755)	1519068..1520177	-	1110	WP_224558030.1	IS110 family transposase	-
LOE41_RS07775 (LOE41_07760)	1520270..1520713	-	444	WP_224557760.1	hypothetical protein	-
LOE41_RS07780 (LOE41_07765)	1520901..1521164	+	264	WP_224557762.1	BrnT family toxin	Toxin
LOE41_RS07785 (LOE41_07770)	1521151..1521366	+	216	WP_224557764.1	CopG family antitoxin	Antitoxin
LOE41_RS07790 (LOE41_07775)	1521719..1521943	-	225	WP_224557765.1	hypothetical protein	-
LOE41_RS07795 (LOE41_07780)	1522111..1522491	-	381	WP_224557766.1	helix-turn-helix domain-containing protein	-
LOE41_RS07800 (LOE41_07785)	1522672..1523643	-	972	WP_224557767.1	hypothetical protein	-
LOE41_RS07805 (LOE41_07790)	1523652..1524596	-	945	WP_230060925.1	integrase arm-type DNA-binding domain-containing protein	-
LOE41_RS07815 (LOE41_07800)	1524974..1525672	-	699	WP_037931342.1	SURF1 family protein	-
LOE41_RS07820 (LOE41_07805)	1525669..1526034	-	366	WP_028957811.1	cytochrome o ubiquinol oxidase subunit IV	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1512520..1524623	12103

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-80)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10254.52 Da
• Isoelectric Point: 5.6550

>T224450 WP_224557762.1 NZ_CP086764:1520901-1521164 [Pseudosulfitobacter pseudonitzschiae]
MKFEYDPDKSAGNKAKHGIDFDEAQALWEDPWLLEAPARTDDEPRFFAIGMIKERHWSAVCTYRGDIIRIISVRRARKQE
IEHYEST

Nucleotide

• Length: 264 bp

>T224450 NZ_CP120329:c4488505-4488131 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGGTCAAAGGGTCTGCGCTTTTTGATACCAATATCCTCATCGACCTGTTTAGCGGTCGAATTGAAGCGAAACATGCGCT
GGAAGCGTATCCGCCACAGAATGCGATTAGCCTTATCACATGGATGGAAGTGATGGTGGGTGCTAAGAAATATCATCAGG
AAAACCGCACGCGCATTGCATTAAGTGCGTTCAATATTATTGGCGTAACACAGGAAATCGCGGAGCGAAGCGTCATTGTC
AGGCAGGAGTATGGCATGAAACTCCCGGATGCGATCATTCTGGCCACTGCACAAGTCCATCGTTGTGAGCTTGTGACGCG
TAATACAAAAGACTTTGCCGATATTCCGGGAGTGATCACGCCGTATCACTTGTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8265.40 Da
• Isoelectric Point: 10.6538

>AT224450 WP_224557764.1 NZ_CP086764:1521151-1521366 [Pseudosulfitobacter pseudonitzschiae]
MKAREFDQKFDTGENVSDLVDWSKARRTNTQTKRVNVDFPSWVVSGLDQKAKQLGVTRQSLIKLWIAERLQ

Nucleotide

• Length: 216 bp

>AT224450 NZ_CP120329:c4488747-4488505 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGACGATGTGATTAAACGGCTTGACGATCTCAAGGT
GCAACGCAACCTACCTCGTGCAGAACTCTTGCGGGAGGCCGTTGAGCAGTATCTGGAAAGACAGGATCGGGCTGAGACCA
CAATTTCGAAAGCATTGGGGCTCTGGCAAGGCTGTGAGGAAGATGGCGTTGAATACCAACGTAAACTTCGTGAGGAATGG
TAA