Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 121575..122227 Replicon plasmid pAZS099-NDM-IMP
Accession NZ_CP086762
Organism Klebsiella pneumoniae strain AZS099

Toxin (Protein)


Gene name vagD Uniprot ID A0A8A2Q2K1
Locus tag LON84_RS25870 Protein ID WP_017901321.1
Coordinates 121575..122000 (-) Length 142 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A853H7M9
Locus tag LON84_RS25875 Protein ID WP_001261275.1
Coordinates 121997..122227 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LON84_RS25835 (LON84_25840) 117284..117400 + 117 Protein_123 transposase -
LON84_RS25840 (LON84_25845) 117525..117695 - 171 Protein_124 LysR family transcriptional regulator -
LON84_RS25845 (LON84_25850) 117706..117933 + 228 Protein_125 IS3 family transposase -
LON84_RS25850 (LON84_25855) 118025..119581 - 1557 WP_025999314.1 sensor domain-containing diguanylate cyclase -
LON84_RS25855 (LON84_25860) 119768..119941 - 174 Protein_127 nuclease -
LON84_RS25860 (LON84_25865) 119994..120530 - 537 Protein_128 integrase core domain-containing protein -
LON84_RS25865 (LON84_25870) 120589..121557 - 969 WP_102097429.1 IS5 family transposase -
LON84_RS25870 (LON84_25875) 121575..122000 - 426 WP_017901321.1 type II toxin-antitoxin system VapC family toxin Toxin
LON84_RS25875 (LON84_25880) 121997..122227 - 231 WP_001261275.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LON84_RS25880 (LON84_25885) 122480..125056 - 2577 WP_017901322.1 nuclease domain-containing protein -
LON84_RS25885 (LON84_25890) 125059..126882 - 1824 WP_247095423.1 AAA family ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaIMP-4 / blaSHV-12 / blaNDM-1 / aph(3'')-Ib / aph(6)-Id / sul2 htpB 1..296148 296148
- flank IS/Tn - - 120589..121557 968


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 142 a.a.        Molecular weight: 15412.91 Da        Isoelectric Point: 7.1364

>T224449 WP_017901321.1 NZ_CP086762:c122000-121575 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVGFVE

Download         Length: 426 bp

>T224449 NZ_CP120329:c4083189-4082905 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTCGCGGTCGGTAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8707.72 Da        Isoelectric Point: 4.3410

>AT224449 WP_001261275.1 NZ_CP086762:c122227-121997 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFDP

Download         Length: 231 bp

>AT224449 NZ_CP120329:c4083421-4083179 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8A2Q2K1

Antitoxin

Source ID Structure
AlphaFold DB A0A853H7M9

References