Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 53143..54053 Replicon plasmid pAZS099-NDM-IMP
Accession NZ_CP086762
Organism Klebsiella pneumoniae strain AZS099

Toxin (Protein)


Gene name - Uniprot ID R4YBC5
Locus tag LON84_RS25515 Protein ID WP_004144067.1
Coordinates 53583..54053 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6A8PHD0
Locus tag LON84_RS25510 Protein ID WP_023287184.1
Coordinates 53143..53586 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LON84_RS25490 (LON84_25495) 48559..49308 + 750 Protein_54 IS5 family transposase -
LON84_RS25495 (LON84_25500) 49337..50305 + 969 WP_000654811.1 IS5 family transposase -
LON84_RS25500 (LON84_25505) 50501..51847 + 1347 WP_227532884.1 Mov34/MPN/PAD-1 family protein -
LON84_RS25505 (LON84_25510) 51900..53042 + 1143 Protein_57 translesion error-prone DNA polymerase V subunit UmuC -
LON84_RS25510 (LON84_25515) 53143..53586 + 444 WP_023287184.1 DUF2384 domain-containing protein Antitoxin
LON84_RS25515 (LON84_25520) 53583..54053 + 471 WP_004144067.1 RES family NAD+ phosphorylase Toxin
LON84_RS25520 (LON84_25525) 54140..55135 + 996 WP_077254794.1 IS5 family transposase -
LON84_RS25525 (LON84_25530) 55137..56105 + 969 WP_023279773.1 IS5 family transposase -
LON84_RS25530 (LON84_25535) 56281..57648 + 1368 WP_017900870.1 formimidoylglutamate deiminase -
LON84_RS25535 (LON84_25540) 58173..58708 + 536 Protein_63 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaIMP-4 / blaSHV-12 / blaNDM-1 / aph(3'')-Ib / aph(6)-Id / sul2 htpB 1..296148 296148
- inside IScluster/Tn - - 46415..66224 19809


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17573.83 Da        Isoelectric Point: 4.5152

>T224447 WP_004144067.1 NZ_CP086762:53583-54053 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T224447 NZ_CP120329:c2353434-2353150 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGACTTATAAGCTGGCATTTAACGAATCAGCACTAAAAGAGTGGAAAAAACTGGGTCATACCATACAGGAACAGTTTAA
AAAAAAGCTACGGAAACGGCTCGAAAACCCTCGGGTTCCTGCTTCGCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGGCTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAATGACACGGCACAGAAGCTAA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16522.84 Da        Isoelectric Point: 10.3013

>AT224447 WP_023287184.1 NZ_CP086762:53143-53586 [Klebsiella pneumoniae]
MKTFSLSSSPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT224447 NZ_CP120329:c2353672-2353424 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACAGAACTGAAACGTGATCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGCAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A384IED8

Antitoxin

Source ID Structure
AlphaFold DB A0A6A8PHD0

References