Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5108828..5109453 Replicon chromosome
Accession NZ_CP086761
Organism Klebsiella pneumoniae strain AZS099

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LON84_RS24855 Protein ID WP_002882817.1
Coordinates 5108828..5109211 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LON84_RS24860 Protein ID WP_004150355.1
Coordinates 5109211..5109453 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LON84_RS24840 (5106194) 5106194..5107096 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LON84_RS24845 (5107093) 5107093..5107728 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LON84_RS24850 (5107725) 5107725..5108654 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LON84_RS24855 (5108828) 5108828..5109211 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LON84_RS24860 (5109211) 5109211..5109453 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LON84_RS24865 (5109658) 5109658..5110575 + 918 WP_023302328.1 alpha/beta hydrolase -
LON84_RS24870 (5110589) 5110589..5111530 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LON84_RS24875 (5111575) 5111575..5112012 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LON84_RS24880 (5112009) 5112009..5112869 - 861 WP_004146232.1 virulence factor BrkB family protein -
LON84_RS24885 (5112863) 5112863..5113462 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T224446 WP_002882817.1 NZ_CP086761:c5109211-5108828 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T224446 NZ_CP120329:1983463-1983565 [Salmonella enterica subsp. enterica serovar Muenchen]
GCCAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT224446 WP_004150355.1 NZ_CP086761:c5109453-5109211 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT224446 NZ_CP120329:c1983567-1983424 [Salmonella enterica subsp. enterica serovar Muenchen]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTGGCTCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References