Detailed information of TA system TA224443

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 4545630..4546423
• Replicon: chromosome
• Accession: NZ_CP086761
• Organism: Klebsiella pneumoniae strain AZS099

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: LON84_RS22240
• Protein ID: WP_200493556.1
• Coordinates: 4545630..4546019 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: LON84_RS22245
• Protein ID: WP_200493555.1
• Coordinates: 4546058..4546423 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LON84_RS22225 (4541715)	4541715..4543394	+	1680	WP_023302424.1	glycoside hydrolase family 43 protein	-
LON84_RS22230 (4543477)	4543477..4544115	-	639	WP_002887060.1	HNH endonuclease	-
LON84_RS22235 (4544295)	4544295..4545164	+	870	WP_023302423.1	HNH endonuclease	-
LON84_RS22240 (4545630)	4545630..4546019	-	390	WP_200493556.1	TA system toxin CbtA family protein	Toxin
LON84_RS22245 (4546058)	4546058..4546423	-	366	WP_200493555.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
LON84_RS22250 (4546449)	4546449..4546670	-	222	WP_200493554.1	DUF987 family protein	-
LON84_RS22255 (4546667)	4546667..4547209	-	543	WP_200493553.1	DNA repair protein RadC	-
LON84_RS22260 (4547222)	4547222..4547665	-	444	WP_200493552.1	antirestriction protein	-
LON84_RS22265 (4547696)	4547696..4548517	-	822	WP_200493551.1	DUF932 domain-containing protein	-
LON84_RS22270 (4548616)	4548616..4548840	-	225	WP_200493550.1	DUF905 domain-containing protein	-
LON84_RS22275 (4548912)	4548912..4549361	-	450	WP_200493549.1	IrmA family protein	-
LON84_RS22280 (4549358)	4549358..4549810	-	453	WP_200493548.1	hypothetical protein	-
LON84_RS22285 (4549847)	4549847..4550416	-	570	WP_200493559.1	hypothetical protein	-
LON84_RS22290 (4550416)	4550416..4551120	-	705	WP_200493547.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (4-112)

Antitoxin

• Antitoxin CbeA (17-117)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14548.67 Da
• Isoelectric Point: 9.6832

>T224443 WP_200493556.1 NZ_CP086761:c4546019-4545630 [Klebsiella pneumoniae]
MQKISSHPIRANQPCLSPIEIWQRLLTHLLSQHYGLTLNDTPFSNDSTIQEHIDAGVSLSDAVNFLVEKYELERIDRDGC
TVMEKSPFITSINILRARKASGLMKRSSHKTVTRVTAGHHQQLKITKGT

Nucleotide

• Length: 390 bp

>T224443 NZ_CP120329:826332-826745 [Salmonella enterica subsp. enterica serovar Muenchen]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTGA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13561.21 Da
• Isoelectric Point: 4.5927

>AT224443 WP_200493555.1 NZ_CP086761:c4546423-4546058 [Klebsiella pneumoniae]
MNNHSESGTTPENPTCQQWGLKSTITPCFGARLVQEDCRIHFLADRAGFNGAFSDNDTLRLDQAFPLMLKQLELMLTSGE
LNPRYQHCVTLYHNGLTCEADTLGSCGYVYIAIYPEQTEPQ

Nucleotide

• Length: 366 bp

>AT224443 NZ_CP120329:826085-826351 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA