Detailed information of TA system TA224442

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 4518211..4519021
• Replicon: chromosome
• Accession: NZ_CP086761
• Organism: Klebsiella pneumoniae strain AZS099

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: A0A332NV98
• Locus tag: LON84_RS22110
• Protein ID: WP_014908042.1
• Coordinates: 4518211..4518744 (-)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: -
• Locus tag: LON84_RS22115
• Protein ID: WP_023343051.1
• Coordinates: 4518755..4519021 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LON84_RS22105 (4517042)	4517042..4518163	+	1122	WP_012737335.1	cupin domain-containing protein	-
LON84_RS22110 (4518211)	4518211..4518744	-	534	WP_014908042.1	type II toxin-antitoxin system toxin KacT	Toxin
LON84_RS22115 (4518755)	4518755..4519021	-	267	WP_023343051.1	type II toxin-antitoxin system antitoxin KacA	Antitoxin
LON84_RS22120 (4519124)	4519124..4520557	-	1434	WP_023343050.1	Cu(+)/Ag(+) sensor histidine kinase	-
LON84_RS22125 (4520547)	4520547..4521230	-	684	WP_002887273.1	copper response regulator transcription factor CusR	-
LON84_RS22130 (4521403)	4521403..4522788	+	1386	WP_024623207.1	efflux transporter outer membrane subunit	-
LON84_RS22135 (4522806)	4522806..4523150	+	345	WP_004192220.1	cation efflux system protein CusF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (87-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 19795.68 Da
• Isoelectric Point: 5.2614

>T224442 WP_014908042.1 NZ_CP086761:c4518744-4518211 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGLGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Nucleotide

• Length: 534 bp

>T224442 NZ_CP120329:206118-206603 [Salmonella enterica subsp. enterica serovar Muenchen]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTCGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGCGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGCCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10021.46 Da
• Isoelectric Point: 10.5807

>AT224442 WP_023343051.1 NZ_CP086761:c4519021-4518755 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRRVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Nucleotide

• Length: 267 bp

>AT224442 NZ_CP120329:205843-206130 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGCTATACAAGGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCAGGCTGCCGAGAAAGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCGGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: A0A332NV98