Detailed information of TA system TA224441

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3905464..3906083
• Replicon: chromosome
• Accession: NZ_CP086761
• Organism: Klebsiella pneumoniae strain AZS099

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: R8WYV2
• Locus tag: LON84_RS19140
• Protein ID: WP_002892050.1
• Coordinates: 3905865..3906083 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: LON84_RS19135
• Protein ID: WP_200493468.1
• Coordinates: 3905464..3905838 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LON84_RS19125 (3900616)	3900616..3901809	+	1194	WP_004177236.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
LON84_RS19130 (3901832)	3901832..3904978	+	3147	WP_002892069.1	multidrug efflux RND transporter permease subunit AcrB	-
LON84_RS19135 (3905464)	3905464..3905838	+	375	WP_200493468.1	Hha toxicity modulator TomB	Antitoxin
LON84_RS19140 (3905865)	3905865..3906083	+	219	WP_002892050.1	HHA domain-containing protein	Toxin
LON84_RS19145 (3906242)	3906242..3906808	+	567	WP_002892030.1	maltose O-acetyltransferase	-
LON84_RS19150 (3906780)	3906780..3906920	-	141	WP_004147370.1	hypothetical protein	-
LON84_RS19155 (3906941)	3906941..3907411	+	471	WP_002892026.1	YlaC family protein	-
LON84_RS19160 (3907386)	3907386..3908837	-	1452	WP_023302231.1	PLP-dependent aminotransferase family protein	-
LON84_RS19165 (3908938)	3908938..3909636	+	699	WP_002892021.1	GNAT family protein	-
LON84_RS19170 (3909633)	3909633..3909773	-	141	WP_002892018.1	type B 50S ribosomal protein L36	-
LON84_RS19175 (3909773)	3909773..3910036	-	264	WP_002892011.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-115)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8628.00 Da
• Isoelectric Point: 8.9008

>T224441 WP_002892050.1 NZ_CP086761:3905865-3906083 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Nucleotide

• Length: 219 bp

>T224441 NZ_CP120328:124387-124692 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTACCGCCTGTTTGTGGATGTGCAGAGCGACATCGTCGATAC
CCCTGGCCGGCGAATGGTGATCCCGCTGGCCAGTGCCCGTCTGCTGTCGGATAAGGTCTCCCGCGAACTTTATCCGGTGG
TGCATATCGGGGATGAGAGCTGGCGTCTGATGACCACGGATATGGCCAGTGTCCCGGCCTCATTCATCGGGGAAGAGGTG
GCCGAGCTCAGCCACAGGGAGAACGACATCAAAAACGCCATTAACCTGATGTTCTGGGGGATCTGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14367.12 Da
• Isoelectric Point: 5.5824

>AT224441 WP_200493468.1 NZ_CP086761:3905464-3905838 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRKNPASLSC

Nucleotide

• Length: 375 bp

>AT224441 NZ_CP120328:124167-124385 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGAAGCAGCGTATTACTGTCACCATAGACAGTGACAGCTATCAGTTACTCAAGTCCGCAGACGTGAATATCTCCGGCCT
GGTAAATACTGCCATGCAGAAGGAGGCCCGCCGTCTGCGTGCGGAACGCTGGCAGGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAGCGAACGGCTCCTTTGCAGACGAAAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A2P8K6F2